BMRB Entry 5314

Title:
Assignment of 1H, 13C, and 15N Resonances of the SH2 Domain of Human Grb14 and Chemical Shift Changes upon Binding a phosphorylated decapeptide
Deposition date:
2002-03-08
Original release date:
2002-12-23
Authors:
Scharf, Paul; Lyons, Barbara
Citation:

Citation: Scharf, Paul; Lyons, Barbara. "Letter to the editor: Assignment of backbone 1H, 13C, and 15N Resonances of the SH2 Domain of Human Grb14"  J. Biomol. NMR 24, 275-276 (2002).

Assembly members:

Assembly members:
Grb14, polymer, 117 residues, Formula weight is not available
pY-766, polymer, 11 residues, 1356 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts726
13C chemical shifts232
15N chemical shifts112

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Grb14 SH2 domain1
2phosphorylated peptide2

Entities:

Entity 1, Grb14 SH2 domain 117 residues - Formula weight is not available

1   GLYSERSERSERALATHRASNMETALAILE
2   HISARGSERGLNPROTRPPHEHISHISLYS
3   ILESERARGASPGLUALAGLNARGLEUILE
4   ILEGLNGLNGLYLEUVALASPGLYVALPHE
5   LEUVALARGASPSERGLNSERASNPROLYS
6   THRPHEVALLEUSERMETSERHISGLYGLN
7   LYSILELYSHISPHEGLNILEILEPROVAL
8   GLUASPASPGLYGLUMETPHEHISTHRLEU
9   ASPASPGLYHISTHRARGPHETHRASPLEU
10   ILEGLNLEUVALGLUPHETYRGLNLEUASN
11   LYSGLYVALLEUPROCYSLYSLEULYSHIS
12   TYRCYSALAARGILEALALEU

Entity 2, phosphorylated peptide 11 residues - 1356 Da.

1   THRSERASNGLNGLUPTRLEUASPLEUSER
2   MET

Related Database Links:

SWISS-PROT Q14449 Q5ICW4
REF XP_001151613 XP_001151683 NP_004481 XP_001151426 NP_001011681
GenBank ACE86896 AAW47246 AAY24176 AAC15861 AAH53559
PDB 2AUG
DBJ BAG35971

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks