BMRB Entry 5284

Title:
1H and 15N Chemical Shift Assignments for Human A54 I-FABP
Deposition date:
2002-02-08
Original release date:
2003-06-25
Authors:
Zhang, Fengli; Luecke, Christian; Baier, Leslie; Sacchettini, James; Hamilton, James
Citation:

Citation: Zhang, Fengli; Luecke, Christian; Baier, Leslie; Sacchettini, James; Hamilton, James. "Solution structure of human intestinal fatty acid binding protein with a naturally-occurring single amino acid substitution (A54T) that is associated with altered lipid metabolism"  Biochemistry 42, 7339-7349 (2003).

Assembly members:

Assembly members:
intestinal fatty acid binding protein, polymer, 131 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-3d

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts955
15N chemical shifts148

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1A54 I-FABP1

Entities:

Entity 1, A54 I-FABP 131 residues - Formula weight is not available

1   ALAPHEASPSERTHRTRPLYSVALASPARG
2   SERGLUASNTYRASPLYSPHEMETGLULYS
3   METGLYVALASNILEVALLYSARGLYSLEU
4   ALAALAHISASPASNLEULYSLEUTHRILE
5   THRGLNGLUGLYASNLYSPHETHRVALLYS
6   GLUSERSERALAPHEARGASNILEGLUVAL
7   VALPHEGLULEUGLYVALTHRPHEASNTYR
8   ASNLEUALAASPGLYTHRGLULEUARGGLY
9   THRTRPSERLEUGLUGLYASNLYSLEUILE
10   GLYLYSPHELYSARGTHRASPASNGLYASN
11   GLULEUASNTHRVALARGGLUILEILEGLY
12   ASPGLULEUVALGLNTHRTYRVALTYRGLU
13   GLYVALGLUALALYSARGILEPHELYSLYS
14   ASP

Samples:

sample_1: intestinal fatty acid binding protein3 – 4 mM

sample_2: intestinal fatty acid binding protein, [U-15N], 3 – 4 mM

cond_1: pH: 6.5; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1H-1H TOCSYnot availablenot availablenot available
1H-1H NOESYnot availablenot availablenot available
1H-15N HSQCnot availablenot availablenot available
1H-15N HTQCnot availablenot availablenot available
3D 1H-15N TOCSY-HMQCnot availablenot availablenot available
3D 1H-15N NOESY-HMQCnot availablenot availablenot available

Software:

XWINNMR v1.3 - processing

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

BMRB 19727 5285
PDB
GB AAA52417 AAH69466 AAH69617 AAH69625 AAH69637
REF NP_000125 XP_001149448 XP_002815138 XP_003830103 XP_004040374
SP P12104
AlphaFold P12104

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks