BMRB Entry 5284
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5284
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Zhang, Fengli; Luecke, Christian; Baier, Leslie; Sacchettini, James; Hamilton, James. "Solution structure of human intestinal fatty acid binding protein with a
naturally-occurring single amino acid substitution (A54T) that is associated
with altered lipid metabolism" Biochemistry 42, 7339-7349 (2003).
Assembly members:
intestinal fatty acid binding protein, polymer, 131 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-3d
Entity Sequences (FASTA):
intestinal fatty acid binding protein: AFDSTWKVDRSENYDKFMEK
MGVNIVKRKLAAHDNLKLTI
TQEGNKFTVKESSAFRNIEV
VFELGVTFNYNLADGTELRG
TWSLEGNKLIGKFKRTDNGN
ELNTVREIIGDELVQTYVYE
GVEAKRIFKKD
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 955 |
| 15N chemical shifts | 148 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | A54 I-FABP | 1 |
Entities:
Entity 1, A54 I-FABP 131 residues - Formula weight is not available
| 1 | ALA | PHE | ASP | SER | THR | TRP | LYS | VAL | ASP | ARG | ||||
| 2 | SER | GLU | ASN | TYR | ASP | LYS | PHE | MET | GLU | LYS | ||||
| 3 | MET | GLY | VAL | ASN | ILE | VAL | LYS | ARG | LYS | LEU | ||||
| 4 | ALA | ALA | HIS | ASP | ASN | LEU | LYS | LEU | THR | ILE | ||||
| 5 | THR | GLN | GLU | GLY | ASN | LYS | PHE | THR | VAL | LYS | ||||
| 6 | GLU | SER | SER | ALA | PHE | ARG | ASN | ILE | GLU | VAL | ||||
| 7 | VAL | PHE | GLU | LEU | GLY | VAL | THR | PHE | ASN | TYR | ||||
| 8 | ASN | LEU | ALA | ASP | GLY | THR | GLU | LEU | ARG | GLY | ||||
| 9 | THR | TRP | SER | LEU | GLU | GLY | ASN | LYS | LEU | ILE | ||||
| 10 | GLY | LYS | PHE | LYS | ARG | THR | ASP | ASN | GLY | ASN | ||||
| 11 | GLU | LEU | ASN | THR | VAL | ARG | GLU | ILE | ILE | GLY | ||||
| 12 | ASP | GLU | LEU | VAL | GLN | THR | TYR | VAL | TYR | GLU | ||||
| 13 | GLY | VAL | GLU | ALA | LYS | ARG | ILE | PHE | LYS | LYS | ||||
| 14 | ASP |
Samples:
sample_1: intestinal fatty acid binding protein3 – 4 mM
sample_2: intestinal fatty acid binding protein, [U-15N], 3 – 4 mM
cond_1: pH: 6.5; temperature: 310 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H TOCSY | not available | not available | not available |
| 1H-1H NOESY | not available | not available | not available |
| 1H-15N HSQC | not available | not available | not available |
| 1H-15N HTQC | not available | not available | not available |
| 3D 1H-15N TOCSY-HMQC | not available | not available | not available |
| 3D 1H-15N NOESY-HMQC | not available | not available | not available |
Software:
XWINNMR v1.3 - processing
NMR spectrometers:
- Bruker DMX 500 MHz
Related Database Links:
| BMRB | 19727 5285 |
| PDB | |
| GB | AAA52417 AAH69466 AAH69617 AAH69625 AAH69637 |
| REF | NP_000125 XP_001149448 XP_002815138 XP_003830103 XP_004040374 |
| SP | P12104 |
| AlphaFold | P12104 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks