BMRB Entry 51867

Title:
human Insulin Fibrils
Deposition date:
2023-03-03
Original release date:
2024-05-20
Authors:
Suladze, Saba; Sankar, Riddhiman; Reif, Bernd
Citation:

Citation: Suladze, Saba; Sarkar, Riddhiman; Rodina, Natalia; Bokvist, Krister; Krewinkel, Manuel; Scheps, Daniel; Nagel, Norbert; Bardiaux, Benjamin; Reif, Bernd. "Atomic resolution structure of full-length human insulin fibrils"  Proc. Natl. Acad. Sci. U. S. A. 121, e2401458121-e2401458121 (2024).
PubMed: 38809711

Assembly members:

Assembly members:
entity_1, polymer, 21 residues, 2383.700 Da.
entity_2, polymer, 30 residues, 3433.981 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pbr322

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GIVEQCCTSICSLYQLENYC N
entity_2: FVNQHLCGSHLVEALYLVCG ERGFFYTPKT

Data sets:
Data typeCount
13C chemical shifts181
15N chemical shifts40

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Human insulin, Chain A1
2Human insulin, Chain B2

Entities:

Entity 1, Human insulin, Chain A 21 residues - 2383.700 Da.

1   GLYILEVALGLUGLNCYSCYSTHRSERILE
2   CYSSERLEUTYRGLNLEUGLUASNTYRCYS
3   ASN

Entity 2, Human insulin, Chain B 30 residues - 3433.981 Da.

1   PHEVALASNGLNHISLEUCYSGLYSERHIS
2   LEUVALGLUALALEUTYRLEUVALCYSGLY
3   GLUARGGLYPHEPHETYRTHRPROLYSTHR

Samples:

sample_1: Human insulin, Chain A, [U-100% 15N], 92.5 mM; Human insulin, Chain B, [U-100% 15N], 92.5 mM

sample_conditions_1: ionic strength: 0.01 M; pH: 1.9; pressure: 1 atm; temperature: 278 K

Experiments:

NameSampleSample stateSample conditions
2D DARRsample_1isotropicsample_conditions_1
3D NCACXsample_1isotropicsample_conditions_1
3D NCOCXsample_1isotropicsample_conditions_1
3D CONCAsample_1isotropicsample_conditions_1
3D NCACBsample_1isotropicsample_conditions_1

Software:

CcpNMR v2.4.2 - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 750 MHz