BMRB Entry 5149

Title:
NMR Structure of the Second Intracellular Loop of the alpha2A adrenergic receptor: evidence for a Novel Cytoplasmic Helix
Deposition date:
2001-09-13
Original release date:
2002-04-09
Authors:
Chung, Duane; Zuiderweg, Erik; Fowler, Carol; Soyer, Orkun; Mosberg, Henry; Neubig, Richard
Citation:

Citation: Chung, Duane; Zuiderweg, Erik; Fowler, Carol; Soyer, Orkun; Mosberg, Henry; Neubig, Richard. "NMR Structure of the Second Intracellular Loop of the alpha2A adrenergic receptor: evidence for a Novel Cytoplasmic Helix"  Biochemistry 41, 3596-3604 (2002).
PubMed: 11888275

Assembly members:

Assembly members:
D130I mutant T3-I2(D130I) peptide, polymer, 32 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
D130I mutant T3-I2(D130I) peptide: TSSIVHLCAISLIRYWSITQ AIEYNLKRTPRR

Data sets:
Data typeCount
1H chemical shifts241

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1D130I mutant T3-I2(D130I) peptide1

Entities:

Entity 1, D130I mutant T3-I2(D130I) peptide 32 residues - Formula weight is not available

1   THRSERSERILEVALHISLEUCYSALAILE
2   SERLEUILEARGTYRTRPSERILETHRGLN
3   ALAILEGLUTYRASNLEULYSARGTHRPRO
4   ARGARG

Samples:

sample_1: D130I mutant T3-I2(D130I) peptide 0.9 mM; dodecyphosphocholine 460 mM; NaCl 50 mM; NaH2PO4 10 mM; H2O 90%; D2O 10%

cond-1: pH: 4.5 na; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-1H NOESYnot availablenot availablenot available
1H-1H TOCSYnot availablenot availablenot available
1H-1H COSYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian INOVA 800 MHz

Related Database Links:

PDB
GB AFJ06726
REF XP_004332099 XP_010176361