BMRB Entry 5149
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR5149
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Citation: Chung, Duane; Zuiderweg, Erik; Fowler, Carol; Soyer, Orkun; Mosberg, Henry; Neubig, Richard. "NMR Structure of the Second Intracellular Loop of the alpha2A adrenergic
receptor: evidence for a Novel Cytoplasmic Helix" Biochemistry 41, 3596-3604 (2002).
PubMed: 11888275
Assembly members:
D130I mutant T3-I2(D130I) peptide, polymer, 32 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
D130I mutant T3-I2(D130I) peptide: TSSIVHLCAISLIRYWSITQ
AIEYNLKRTPRR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 241 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | D130I mutant T3-I2(D130I) peptide | 1 |
Entities:
Entity 1, D130I mutant T3-I2(D130I) peptide 32 residues - Formula weight is not available
| 1 | THR | SER | SER | ILE | VAL | HIS | LEU | CYS | ALA | ILE | ||||
| 2 | SER | LEU | ILE | ARG | TYR | TRP | SER | ILE | THR | GLN | ||||
| 3 | ALA | ILE | GLU | TYR | ASN | LEU | LYS | ARG | THR | PRO | ||||
| 4 | ARG | ARG |
Samples:
sample_1: D130I mutant T3-I2(D130I) peptide 0.9 mM; dodecyphosphocholine 460 mM; NaCl 50 mM; NaH2PO4 10 mM; H2O 90%; D2O 10%
cond-1: pH: 4.5 na; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H NOESY | not available | not available | not available |
| 1H-1H TOCSY | not available | not available | not available |
| 1H-1H COSY | not available | not available | not available |
Software:
No software information available
NMR spectrometers:
- Varian INOVA 800 MHz