BMRB Entry 5083
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR5083
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Gutierrez-Gonzalez, Luis; Ludwig, Christian; Hohoff, Carsten; Rademacher, Martin; Hanhoff, Thorsten; Rueterjans, Heinz; Spener, Friedrich; Luecke, Christian. "Solution Structure and Backbone Dynamics of Human Epidermal-Type Fatty
Acid-Binding Protein (E-FABP)" Biochem. J. 364, 725-737 (2002).
PubMed: 12049637
Assembly members:
Epidermal-type fatty acid binding protein, polymer, 135 residues, 15164 Da.
STE, non-polymer, 284.477 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3d
Entity Sequences (FASTA):
Epidermal-type fatty acid binding protein: MATVQQLEGRWRLVDSKGFD
EYMKELGVGIALRKMGAMAK
PDCIITCDGKNLTIKTESTL
KTTQFSCTLGEKFEETTADG
RKTQTVCNFTDGALVQHQEW
DGKESTITRKLKDGKLVVEC
VMNNVTCTRIYEKVE
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 152 |
| 1H chemical shifts | 966 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | E-FABP | 1 |
| 2 | stearate | 2 |
Entities:
Entity 1, E-FABP 135 residues - 15164 Da.
| 1 | MET | ALA | THR | VAL | GLN | GLN | LEU | GLU | GLY | ARG | ||||
| 2 | TRP | ARG | LEU | VAL | ASP | SER | LYS | GLY | PHE | ASP | ||||
| 3 | GLU | TYR | MET | LYS | GLU | LEU | GLY | VAL | GLY | ILE | ||||
| 4 | ALA | LEU | ARG | LYS | MET | GLY | ALA | MET | ALA | LYS | ||||
| 5 | PRO | ASP | CYS | ILE | ILE | THR | CYS | ASP | GLY | LYS | ||||
| 6 | ASN | LEU | THR | ILE | LYS | THR | GLU | SER | THR | LEU | ||||
| 7 | LYS | THR | THR | GLN | PHE | SER | CYS | THR | LEU | GLY | ||||
| 8 | GLU | LYS | PHE | GLU | GLU | THR | THR | ALA | ASP | GLY | ||||
| 9 | ARG | LYS | THR | GLN | THR | VAL | CYS | ASN | PHE | THR | ||||
| 10 | ASP | GLY | ALA | LEU | VAL | GLN | HIS | GLN | GLU | TRP | ||||
| 11 | ASP | GLY | LYS | GLU | SER | THR | ILE | THR | ARG | LYS | ||||
| 12 | LEU | LYS | ASP | GLY | LYS | LEU | VAL | VAL | GLU | CYS | ||||
| 13 | VAL | MET | ASN | ASN | VAL | THR | CYS | THR | ARG | ILE | ||||
| 14 | TYR | GLU | LYS | VAL | GLU |
Entity 2, stearate - C18 H36 O2 - 284.477 Da.
| 1 | STE |
Samples:
sample_1: Epidermal-type fatty acid binding protein1.5 – 2.0 mM; STEARIC ACID1.5 – 2.0 mM
sample_2: Epidermal-type fatty acid binding protein, [U-15N], 1.5 – 2.0 mM; STEARIC ACID1.5 – 2.0 mM
cond_1: pH: 5.6; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H TOCSY | not available | not available | cond_1 |
| 1H-1H NOESY | not available | not available | cond_1 |
| 1H-15N HSQC | not available | not available | cond_1 |
| 1H-15N HTQC | not available | not available | cond_1 |
| 1H-15N TOCSY-HSQC | not available | not available | cond_1 |
| 1H-15N NOESY-HSQC | not available | not available | cond_1 |
Software:
XWINNMR v1.3 - data acquisition, data processing
AURELIA v2.5.9 - data analysis
NMR spectrometers:
- Bruker DMX 600 MHz
Related Database Links:
| BMRB | 7107 |
| PDB | |
| DBJ | BAG34797 |
| GenBank | AAA58467 AAH19385 AAH70303 AAP36117 AAX41860 |
| REF | NP_001435 XP_001091773 XP_001127657 XP_001128089 XP_001168193 |
| SWISS-PROT | Q01469 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks