BMRB Entry 50337

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR50337
MolProbity Validation Chart

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Title:
SARS Cov2 nsp7 backbone assignments
Deposition date:
2020-06-22
Original release date:
2021-03-11
Authors:
Tonelli, Marco; Kirchdoerf, Robert N; Henzler-Wildman, Katherine
Citation:

Citation: Tonelli, Marco; Rienstra, Chad; Anderson, Thomas; Kirchdoerf, Robert; Henzler-Wildman, Katherine. "1H, 13C, and 15N backbone and side chain chemical shift assignments of the SARS-CoV-2 non-structural protein 7"  Biomol. NMR Assign. 15, 73-77 (2021).
PubMed: 33219414

Assembly members:

Assembly members:
entity_1, polymer, 84 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: SARS CoV2   Taxonomy ID: 2697049   Superkingdom: Viruses   Kingdom: not available   Genus/species: Betacoronavirus HCoV-SARS

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET46

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSKMSDVKCTSVVLLSVLQQ LRVESSSKLWAQCVQLHNDI LLAKDTTEAFEKMVSLLSVL LSMQGAVDINKLCEEMLDNR ATLQ

Data sets:
Data typeCount
13C chemical shifts372
15N chemical shifts93
1H chemical shifts625

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1nsp71

Entities:

Entity 1, nsp7 84 residues - Formula weight is not available

1   GLYSERLYSMETSERASPVALLYSCYSTHR
2   SERVALVALLEULEUSERVALLEUGLNGLN
3   LEUARGVALGLUSERSERSERLYSLEUTRP
4   ALAGLNCYSVALGLNLEUHISASNASPILE
5   LEULEUALALYSASPTHRTHRGLUALAPHE
6   GLULYSMETVALSERLEULEUSERVALLEU
7   LEUSERMETGLNGLYALAVALASPILEASN
8   LYSLEUCYSGLUGLUMETLEUASPASNARG
9   ALATHRLEUGLN

Samples:

sample_1: nsp7, [U-100% 13C; U-100% 15N], 1.7 mM; MOPS 10 mM; sodium chloride 150 mM; DTT 2 mM; sodium azide 0.05%

sample_conditions_1: pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
2D 1H-13C-HSQC aliphaticsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D H(C)CH-TOCSY aliphaticsample_1isotropicsample_conditions_1
3D NOESY 1H-13C-HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C-HSQC aromaticsample_1isotropicsample_conditions_1
3D (HB)CB(CGCD)HEsample_1isotropicsample_conditions_1
3D H(C)CH-TOCSY aromaticsample_1isotropicsample_conditions_1
2D NOESY 1H-13C-HSQC aromaticsample_1isotropicsample_conditions_1

Software:

VNMRj - collection

NMRPipe - processing

NMRFAM-SPARKY - chemical shift assignment

I-PINE - chemical shift assignment

NMR spectrometers:

  • Varian VXRS 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks