BMRB Entry 5000

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR5000

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

1H, 15N and 13C Resonance Assignments for the N-Terminal Domain of the MAPK Phosphatase PAC-1

Deposition date:

2000-04-26

Original release date:

2002-05-09

Authors:

Chaturvedi, Gaurav; Zheng, Lei; Zhou, Ming-Ming

Citation:

Citation: Chaturvedi, Gaurav; Farooq, Amjad; Zheng, Lei; Zhou, Ming-Ming. "Letter to the Editor: 1H, 13C and 15N Resonance Assignments of the Catalytic Domain of the MAPK Phosphatase, PAC-1" J. Biomol. NMR 25, 79-80 (2003).

Assembly members:

Assembly members:
MAP Kinase Phosphatase PAC-1, polymer, 141 residues, 15100 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MAP Kinase Phosphatase PAC-1: VEILPYLFLGSCSHSSDLQG LQACGITAVLNVSASCPNHF EGLFRYKSIPVEDNQMVEIS AWFQEAIGFIDWVKNSGGRV LVHSQAGISRSATICLAYLM QSRRVRLDEAFDFVKQRRGV ISPNFSFMGQLLQFETQVLC H

Data sets:

assigned_chemical_shifts
- shift_set-1

Data type	Count
1H chemical shifts	685
13C chemical shifts	300
15N chemical shifts	112

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PAC-1 C-Terminal Domain	1

Entities:

Entity 1, PAC-1 C-Terminal Domain 141 residues - 15100 Da.

1

VAL

GLU

ILE

LEU

PRO

TYR

LEU

PHE

LEU

GLY

2

SER

CYS

SER

HIS

SER

ASP

LEU

GLN

GLY

3

LEU

GLN

ALA

CYS

GLY

ILE

THR

ALA

VAL

LEU

4

ASN

VAL

SER

ALA

SER

CYS

PRO

ASN

HIS

PHE

5

GLU

GLY

LEU

PHE

ARG

TYR

LYS

SER

ILE

PRO

6

VAL

GLU

ASP

ASN

GLN

MET

VAL

GLU

ILE

SER

7

ALA

TRP

PHE

GLN

GLU

ALA

ILE

GLY

PHE

ILE

8

ASP

TRP

VAL

LYS

ASN

SER

GLY

ARG

VAL

9

LEU

VAL

HIS

SER

GLN

ALA

GLY

ILE

SER

ARG

10

SER

ALA

THR

ILE

CYS

LEU

ALA

TYR

LEU

MET

11

GLN

SER

ARG

VAL

ARG

LEU

ASP

GLU

ALA

12

PHE

ASP

PHE

VAL

LYS

GLN

ARG

GLY

VAL

13

ILE

SER

PRO

ASN

PHE

SER

PHE

MET

GLY

GLN

14

LEU

GLN

PHE

GLU

THR

GLN

VAL

LEU

CYS

15

HIS

Samples:

sample_1: MAP Kinase Phosphatase PAC-1, [U-99% 13C; U-99% 15N], 0.4 – 0.6 mM

Ex-cond_1: pH: 8.0; temperature: 298 K; ionic strength: 0.1 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N NOESY	sample_1	not available	Ex-cond_1
1H-15N TOCSY	sample_1	not available	Ex-cond_1
HNCA	sample_1	not available	Ex-cond_1
HN(CO)CA	sample_1	not available	Ex-cond_1
HNCACB	sample_1	not available	Ex-cond_1
HN(COCA)CB	sample_1	not available	Ex-cond_1
HNCO	sample_1	not available	Ex-cond_1
C(CO)NH	sample_1	not available	Ex-cond_1

Software:

No software information available

NMR spectrometers:

Bruker . 500 MHz
Bruker . 600 MHz

BMRB	5552
PDB	1M3G
GB	AAA50779 AAA86112 AAH07771 AAY24222 ACE87048
REF	NP_004409 XP_001111118 XP_002757414 XP_002811694 XP_003281084
SP	Q05923
AlphaFold	Q05923