BMRB Entry 4939

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PDB ID: 1fu9
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4939
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structures of Two CCHC Zinc Fingers from the FOG Family Protein U-shaped that Mediate Protein-Protein Interactions

Deposition date:

2001-01-13

Original release date:

2001-01-23

Authors:

Kowalski, K.; Mackay, J.

Citation:

Citation: Liew, C.; Kowalski, K.; Fox, A.; Newton, A.; Sharpe, B.; Crossley, M.; Mackay, J.. "Solution Structures of Two CCHC Zinc Fingers from the FOG Family Protein U-shaped that Mediate Protein-Protein Interactions" Structure 8, 1157-1166 (2000).

Assembly members:

Assembly members:
U-shaped transcriptional cofactor, polymer, 36 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
U-shaped transcriptional cofactor: GSAAEVMKKYCSTCDISFNY VKTYLAHKQFYCKNKP

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	210
15N chemical shifts	34

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	U-shaped transcriptional cofactor	1
2	ZINC ION	2

Entities:

Entity 1, U-shaped transcriptional cofactor 36 residues - Formula weight is not available

1

GLY

SER

ALA

GLU

VAL

MET

LYS

TYR

2

CYS

SER

THR

CYS

ASP

ILE

SER

PHE

ASN

TYR

3

VAL

LYS

THR

TYR

LEU

ALA

HIS

LYS

GLN

PHE

4

TYR

CYS

LYS

ASN

LYS

PRO

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: U-shaped transcriptional cofactor 0.4 mM; ZnSO4 0.6 mM; TCEP 0.6 mM

sample_2: U-shaped transcriptional cofactor, [U-15N], 0.2 mM; ZnSO4 0.3 mM; TCEP 0.3 mM

sample_cond_1: pH: 5.0; temperature: 293 K; ionic strength: 0.6 mM; pressure: 1 atm

sample_cond_2: pH: 5.0; temperature: 293 K; ionic strength: 0.3 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	not available	not available	not available
HNHA	not available	not available	not available
HNHB	not available	not available	not available

Software:

XWINNMR v2.1 - data processing

XEASY v1.3.13 - data analysis

DYANA v1.5 - structure solution

CNS v0.5 - refinement

NMR spectrometers:

Bruker DRX 600 MHz

REF	XP_002077649
GenBank	EDX03234
PDB	1FU9