BMRB Entry 4925
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4925
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Lin, Yingxi; Dotsch, Volker; Wintner, T.; Peariso, Katrina; Myers, Lawrence; Penner-Hahn, James; Verdine, Gregory; Wagner, Gerhard. "Structural Basis for the Functional switch of the E. Coli Ada Protein" Biochemistry 40, 4261-4271 (2001).
PubMed: 11284682
Assembly members:
N-Ada10, polymer, 92 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
N-Ada10: MKKATCLTDDQRWQSVLARD
PNADGEFVFAVRTTGIFCRP
SCRARHALRENVSFYANASE
ALAAGFRPCKRCQPDKANPR
QHRLDKITHACR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 556 |
| 13C chemical shifts | 85 |
| 15N chemical shifts | 90 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | N-Ada10 subunit 1 | 1 |
| 2 | Zn2+ | 2 |
Entities:
Entity 1, N-Ada10 subunit 1 92 residues - Formula weight is not available
| 1 | MET | LYS | LYS | ALA | THR | CYS | LEU | THR | ASP | ASP | ||||
| 2 | GLN | ARG | TRP | GLN | SER | VAL | LEU | ALA | ARG | ASP | ||||
| 3 | PRO | ASN | ALA | ASP | GLY | GLU | PHE | VAL | PHE | ALA | ||||
| 4 | VAL | ARG | THR | THR | GLY | ILE | PHE | CYS | ARG | PRO | ||||
| 5 | SER | CYS | ARG | ALA | ARG | HIS | ALA | LEU | ARG | GLU | ||||
| 6 | ASN | VAL | SER | PHE | TYR | ALA | ASN | ALA | SER | GLU | ||||
| 7 | ALA | LEU | ALA | ALA | GLY | PHE | ARG | PRO | CYS | LYS | ||||
| 8 | ARG | CYS | GLN | PRO | ASP | LYS | ALA | ASN | PRO | ARG | ||||
| 9 | GLN | HIS | ARG | LEU | ASP | LYS | ILE | THR | HIS | ALA | ||||
| 10 | CYS | ARG |
Entity 2, Zn2+ - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: N-Ada10, [U-15N; U-13C], 2 – 3 mM
Ex-cond1: pH: 6.4; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-15N NOESY | sample_1 | not available | Ex-cond1 |
| 1H-15N TOCSY | sample_1 | not available | Ex-cond1 |
| 2D NOESY | sample_1 | not available | Ex-cond1 |
| HCCH-TOCSY | sample_1 | not available | Ex-cond1 |
Software:
No software information available
NMR spectrometers:
- Varian Unity-plus 750 MHz
- Varian Unity-plus 450 MHz
- Bruker AMX 500 MHz
- Bruker AMX 600 MHz
Related Database Links:
| REF | YP_670153 YP_408500 YP_311153 NP_837823 NP_708108 |
| GenBank | ABB66672 AAZ88918 AAP17632 AAN43815 AAA23413 |
| PDB | |
| BMRB | 6605 |
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