BMRB Entry 4885

Title:
Backbone 1H, 15N and 13C Resonance Assignments of the NTPase Subdomain of the Hepatitis C Virus NS3 RNA Helicase
Deposition date:
2000-10-25
Original release date:
2001-11-14
Authors:
Liu, Dingjiang; Wyss, Daniel
Citation:

Citation: Liu, Dingjiang; Wyss, Daniel. "Backbone 1H, 15N and 13C Resonance Assignments of the NTPase Subdomain of the Hepatitis C Virus NS3 RNA Helicase"  J. Biomol. NMR 19, 283-284 (2001).
PubMed: 11330818

Assembly members:

Assembly members:
HCV helicase, polymer, 144 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: hepatitis C virus   Taxonomy ID: 31646   Superkingdom: viruses   Kingdom: not available   Genus/species: viruses hepatitis C virus

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts119
13C chemical shifts356
15N chemical shifts119

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HCV helicase NTPase domain1

Entities:

Entity 1, HCV helicase NTPase domain 144 residues - Formula weight is not available

1   SERPROVALPHETHRASPASNSERSERPRO
2   PROALAVALPROGLNSERPHEGLNVALALA
3   HISLEUHISALAPROTHRGLYSERGLYLYS
4   SERTHRLYSVALPROALAALATYRALAALA
5   GLNGLYTYRLYSVALLEUVALLEUASNPRO
6   SERVALALAALATHRLEUGLYPHEGLYALA
7   TYRMETSERLYSALAHISGLYVALASPPRO
8   ASNILEARGTHRGLYVALARGTHRILETHR
9   THRGLYSERPROILETHRTYRSERTHRTYR
10   GLYLYSPHELEUALAASPGLYGLYCYSSER
11   GLYGLYALATYRASPILEILEILECYSASP
12   GLUCYSHISSERTHRASPALATHRSERILE
13   LEUGLYILEGLYTHRVALLEUASPGLNALA
14   GLUTHRALAGLYALAARGLEUVALVALLEU
15   ALATHRALATHR

Samples:

sample_1: HCV helicase, [U-15N; U-13C; U-2H], 0.075 – 0.5 mM

condition_1: pH: 7.4; temperature: 293 K; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESY-HSQCnot availablenot availablenot available
1H-15N TOCSY-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCOCAnot availablenot availablenot available
HNCOCACBnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNCOnot availablenot availablenot available

Software:

FELIX v98 -

NMRView v4.1.2 -

NMR spectrometers:

  • Varian UNITYplus 600 MHz

Related Database Links:

PDB
DBJ BAA01582 BAA03178 BAA08120 BAA09073 BAA09075
EMBL CAC03609 CAJ20150 CAJ20155 CAJ20158 CAJ20165
GB AAA45534 AAA45676 AAA45677 AAB02124 AAB02125
REF NP_671491 NP_803144
SP P26664 P27958 Q03463
AlphaFold Q03463 P26664 P27958

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks