BMRB Entry 4834

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4834

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Title:
Backbone (1H, 15N, 13C) Resonance Assignments of a 21 kDa construct of S. aureus Peptide Deformylase
Deposition date:
2000-09-18
Original release date:
2001-02-14
Authors:
Scahill, Terrence; Kloosterman, David; Cialdella, Joyce; Deibel, Martin; Marshall, Vincent; Yem, Anthony
Citation:

Citation: Scahill, Terrence; Kloosterman, David; Cialdella, Joyce; Deibel, Martin; Marshall, Vincent; Yem, Anthony. "Letter to the Editor: Backbone (1H, 15N, 13C) resonance assignments of a 21 kDa construct of S. aureus peptide deformylase"  J. Biomol. NMR 19, 81-82 (2001).

Assembly members:

Assembly members:
Staphylococcal aureus peptide deformylase, polymer, 189 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 1280   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Staphylococcal aureus peptide deformylase: MLTMKDIIRDGHPTLRQKAA ELELPLTKEEKETLIAMREF LVNSQDEEIAKRYGLRSGVG LAAPQINISKRMIAVLIPDD GSGKSYDYMLVNPKIVSHSV QEAYLPTGEGCLSVDDNVAG LVHRHNRITIKAKDIEGNDI QLRLKGYPAIVFQHEIDHLN GVMFYDHIDKDHPLQPHTDA VEVHHHHHH

Data sets:
Data typeCount
1H chemical shifts632
13C chemical shifts509
15N chemical shifts166

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1pdf1
2ZN 2+2

Entities:

Entity 1, pdf 189 residues - Formula weight is not available

1   METLEUTHRMETLYSASPILEILEARGASP
2   GLYHISPROTHRLEUARGGLNLYSALAALA
3   GLULEUGLULEUPROLEUTHRLYSGLUGLU
4   LYSGLUTHRLEUILEALAMETARGGLUPHE
5   LEUVALASNSERGLNASPGLUGLUILEALA
6   LYSARGTYRGLYLEUARGSERGLYVALGLY
7   LEUALAALAPROGLNILEASNILESERLYS
8   ARGMETILEALAVALLEUILEPROASPASP
9   GLYSERGLYLYSSERTYRASPTYRMETLEU
10   VALASNPROLYSILEVALSERHISSERVAL
11   GLNGLUALATYRLEUPROTHRGLYGLUGLY
12   CYSLEUSERVALASPASPASNVALALAGLY
13   LEUVALHISARGHISASNARGILETHRILE
14   LYSALALYSASPILEGLUGLYASNASPILE
15   GLNLEUARGLEULYSGLYTYRPROALAILE
16   VALPHEGLNHISGLUILEASPHISLEUASN
17   GLYVALMETPHETYRASPHISILEASPLYS
18   ASPHISPROLEUGLNPROHISTHRASPALA
19   VALGLUVALHISHISHISHISHISHIS

Entity 2, ZN 2+ - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: Staphylococcal aureus peptide deformylase, [95% 13C; 90% 15N], 0.3 – 1.0 mM; ZINC (II) ION0.6 – 2.0 mM

sample_conditions: pH*: 7.4; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1not availablesample_conditions
1H-15N NOESYsample_1not availablesample_conditions
HNCAsample_1not availablesample_conditions
HN(CO)CAsample_1not availablesample_conditions
HNCOsample_1not availablesample_conditions
CBCACONHsample_1not availablesample_conditions
NHCACBsample_1not availablesample_conditions
HBHACONHsample_1not availablesample_conditions

Software:

NMRPipe - visualization and resonance assignments

Felix - visualization and resonance assignments

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAB42188 BAB57253 BAB94839 BAF67229 BAF77966
EMBL CAG40067 CAG42800 CAI80645 CAQ49513 CBI48965
GB AAG02249 AAW37980 ABD21929 ABD30158 ABQ48951
REF NP_371615 NP_374210 NP_645791 WP_000957034 WP_000957036
SP P68825 P68826 P99077 Q5HGZ3 Q6GAC3
AlphaFold P68825 P68826 P99077 Q5HGZ3 Q6GAC3

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks