BMRB Entry 4827

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4827

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Title:
Structural comparison between WT and P25S human cystatin A by NMR spectroscopy. Does this mutation affect the alpha-helix conformation? -- Part I wild type assignments
Deposition date:
2000-09-13
Original release date:
2002-04-08
Authors:
Shimba, Nobuhisa; Kariya, Eri; Tate, Shin-ichi; Kaji, Hiroyuki; Kainosho, Masatsune
Citation:

Citation: Shimba, Nobuhisa; Kariya, Eri; Tate, Shin-ichi; Kaji, Hiroyuki; Kainosho, Masatsune. "Structural comparison between WT and P25S human cystatin A by NMR spectroscopy. Does this mutation affect the alpha-helix conformation?"  J. Struct. Func. Genomics. 1, 26-42 (2001).

Assembly members:

Assembly members:
cystain A, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Homo sapiens   Taxonomy ID: organism   Superkingdom: not available   Kingdom: Eukaryota   Genus/species: Metazoa Homo

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cystain A: IPGGLSEAKPATPEIQEIVD KVKPQLEEKTNETYGKLEAV QYKTQVVAGTNYYIKVRAGD NKYLHLKVFKSLPGQNEDLV LTGYQVDKNKDDELTGF

Data sets:
Data typeCount
13C chemical shifts274
1H chemical shifts652
15N chemical shifts102

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cystatin A1

Entities:

Entity 1, cystatin A 97 residues - Formula weight is not available

1   ILEPROGLYGLYLEUSERGLUALALYSPRO
2   ALATHRPROGLUILEGLNGLUILEVALASP
3   LYSVALLYSPROGLNLEUGLUGLULYSTHR
4   ASNGLUTHRTYRGLYLYSLEUGLUALAVAL
5   GLNTYRLYSTHRGLNVALVALALAGLYTHR
6   ASNTYRTYRILELYSVALARGALAGLYASP
7   ASNLYSTYRLEUHISLEULYSVALPHELYS
8   SERLEUPROGLYGLNASNGLUASPLEUVAL
9   LEUTHRGLYTYRGLNVALASPLYSASNLYS
10   ASPASPGLULEUTHRGLYPHE

Samples:

sample_1: cystain A, [U-13C; U-15N], 1.4 mM

sample_2: cystain A, [U-15N], 1.4 mM

sample_3: cystain A 1.4 mM

Ex-cond_1: pH: 4.0; temperature: 310 K; ionic strength: 0.01 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availableEx-cond_1
1H-1H HOHAHAnot availablenot availableEx-cond_1
HNCOnot availablenot availableEx-cond_1
HNCAnot availablenot availableEx-cond_1
CBCA(CO)NHnot availablenot availableEx-cond_1
CBCANHnot availablenot availableEx-cond_1
HBHA(CO)NHnot availablenot availableEx-cond_1
HCCH-TOCSYnot availablenot availableEx-cond_1
HCCH-COSYnot availablenot availableEx-cond_1

Software:

nmrPipe -

PIPP -

NMR spectrometers:

  • Bruker DRX 500 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 4828
PDB
DBJ BAA00307 BAA13609 BAA87858 BAF83997
EMBL CAA29398 CAG33195
GB AAA72785 AAH10379 AIC54242 EAW79489
REF NP_005204 XP_001166441 XP_003825238 XP_004036218
SP P01040
AlphaFold P01040

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks