BMRB Entry 4818

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4818

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Title:
1H, 15N and 13C Resonance Assignments for the N-Terminal Domain of the MAPK Phosphatase MKP3
Deposition date:
2000-09-04
Original release date:
2001-11-14
Authors:
Farooq, Amjad; Zeng, Lei; Zhou, Ming-Ming
Citation:

Citation: Farooq, Amjad; Zeng, Lei; Zhou, Ming-Ming. "1H, 13C and 15N Resonance Assignments of the ERK2 Binding Domain of the MAPK Phosphatase MKP-3"  J. Biomol. NMR 19, 195-196 (2001).
PubMed: 11256818

Assembly members:

Assembly members:
Mitogen-Activated Protein Kinase Phosphatase 3, polymer, 154 residues, 17000 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Mitogen-Activated Protein Kinase Phosphatase 3: MIDTLRPVPFASEMAISKTV AWLNEQLELGNERLLLMDCR PQELYESSHIESAINVAIPG IMLRRLQKGNLPVRALFTRG EDRDRFTRRCGTDTVVLYDE SSSDWNENTGGESLLGLLLK KLKDEGCRAFYLEGGFSKFQ AEFSLHCETNLDGS

Data sets:
Data typeCount
1H chemical shifts910
13C chemical shifts498
15N chemical shifts160

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MKP-3 N-Terminal Domain1

Entities:

Entity 1, MKP-3 N-Terminal Domain 154 residues - 17000 Da.

1   METILEASPTHRLEUARGPROVALPROPHE
2   ALASERGLUMETALAILESERLYSTHRVAL
3   ALATRPLEUASNGLUGLNLEUGLULEUGLY
4   ASNGLUARGLEULEULEUMETASPCYSARG
5   PROGLNGLULEUTYRGLUSERSERHISILE
6   GLUSERALAILEASNVALALAILEPROGLY
7   ILEMETLEUARGARGLEUGLNLYSGLYASN
8   LEUPROVALARGALALEUPHETHRARGGLY
9   GLUASPARGASPARGPHETHRARGARGCYS
10   GLYTHRASPTHRVALVALLEUTYRASPGLU
11   SERSERSERASPTRPASNGLUASNTHRGLY
12   GLYGLUSERLEULEUGLYLEULEULEULYS
13   LYSLEULYSASPGLUGLYCYSARGALAPHE
14   TYRLEUGLUGLYGLYPHESERLYSPHEGLN
15   ALAGLUPHESERLEUHISCYSGLUTHRASN
16   LEUASPGLYSER

Samples:

sample_1: Mitogen-Activated Protein Kinase Phosphatase 3, [U-99% 13C; U-99% 15N], 0.4 – 0.6 mM

Ex-cond_1: pH: 6.0; temperature: 298 K; ionic strength: 0.1 M

Experiments:

NameSampleSample stateSample conditions
1H-15N NOESYsample_1not availableEx-cond_1
1H-15N TOCSYsample_1not availableEx-cond_1
HNCAsample_1not availableEx-cond_1
HN(CO)CAsample_1not availableEx-cond_1
HNCACBsample_1not availableEx-cond_1
HN(COCA)CBsample_1not availableEx-cond_1
HNCOsample_1not availableEx-cond_1
C(CO)NHsample_1not availableEx-cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker . 500 MHz
  • Bruker . 600 MHz

Related Database Links:

PDB
DBJ BAA31968 BAA34369 BAD38886 BAG70079 BAG70207
EMBL CAA63813
GB AAH03143 AAH03562 AAH05047 AAH37236 AAI05539
REF NP_001039660 NP_001244721 NP_001254771 NP_001937 XP_001165821
SP Q16828 Q2KJ36
TPG DAA30092
AlphaFold Q16828 Q2KJ36

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks