BMRB Entry 4791

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PDB ID: 1jr6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4791
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Sequence-specific 1H, 15N and 13C Resonance Assignments for an Engineered Arginine-rich Domain of the Hepatitis C Virus NS3 RNA Helicase

Deposition date:

2000-07-21

Original release date:

2001-05-03

Authors:

Liu, Dingjiang; Wyss, Daniel

Citation:

Citation: Liu, Dingjiang; Wyss, Daniel. "Letter to the Editor: Sequence-specific 1H, 15N and 13C Resonance Assignments for an Engineered Arginine-rich Domain of the Hepatitis C Virus NS3 RNA Helicase" J. Biomol. NMR 18, 279-280 (2000).

Assembly members:

Assembly members:
HCV helicase, polymer, 142 residues, Formula weight is not available

Natural source:

Natural source: Common Name: hepatitis C virus Taxonomy ID: 11103 Superkingdom: viruses Kingdom: not available Genus/species: hepatitis C virus hepatitis C virus

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
HCV helicase: GSHMGSVTVPHPNIEEVALS TTGEIPFYGKAIPLEVIKGG RHLIFCHSKKKCDELAAKLV ALGINAVAYYRGLDVSVIPT NGDVVVVATDALMTGFTGDF DSVIDCNTSDGKPQDAVSRT QRRGRTGRGKPGIYRFVAPG ER

Data sets:

assigned_chemical_shifts
- shiftset_1

Data type	Count
13C chemical shifts	577
15N chemical shifts	143
1H chemical shifts	942

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HCV NS3 RNA helicase domain 2	1

Entities:

Entity 1, HCV NS3 RNA helicase domain 2 142 residues - Formula weight is not available

1

GLY

SER

HIS

MET

GLY

SER

VAL

THR

VAL

PRO

2

HIS

PRO

ASN

ILE

GLU

VAL

ALA

LEU

SER

3

THR

GLY

GLU

ILE

PRO

PHE

TYR

GLY

LYS

4

ALA

ILE

PRO

LEU

GLU

VAL

ILE

LYS

GLY

5

ARG

HIS

LEU

ILE

PHE

CYS

HIS

SER

LYS

6

LYS

CYS

ASP

GLU

LEU

ALA

LYS

LEU

VAL

7

ALA

LEU

GLY

ILE

ASN

ALA

VAL

ALA

TYR

8

ARG

GLY

LEU

ASP

VAL

SER

VAL

ILE

PRO

THR

9

ASN

GLY

ASP

VAL

ALA

THR

ASP

10

ALA

LEU

MET

THR

GLY

PHE

THR

GLY

ASP

PHE

11

ASP

SER

VAL

ILE

ASP

CYS

ASN

THR

SER

ASP

12

GLY

LYS

PRO

GLN

ASP

ALA

VAL

SER

ARG

THR

13

GLN

ARG

GLY

ARG

THR

GLY

ARG

GLY

LYS

14

PRO

GLY

ILE

TYR

ARG

PHE

VAL

ALA

PRO

GLY

15

GLU

ARG

Samples:

sample-1: HCV helicase, [U-15N; U-13C], 0.2 – 1.0 mM; KPO4 75 mM; DTT, [U-2H], 5 mM; D2O 99%; AEBSF 0.4 mM; NaN3 0.015%

sample-2: HCV helicase, [U-15N; U-13C], 0.2 – 1.0 mM; KPO4 75 mM; DTT, [U-2H], 5 mM; D2O 5%; AEBSF 0.4 mM; NaN3 0.015%

cond_set_1: pH: 6.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
1H-15N HSQC	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
HNCACB	not available	not available	not available
HNCO	not available	not available	not available
(H)CC(CO)NH-TOCSY	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
15N-edited TOCSY-HSQC	not available	not available	not available

Software:

FELIX v98.0 - data processing

NMRView v4.0.3 - data analysis

NMR spectrometers:

Varian INOVA 500 MHz
Varian INOVA 600 MHz

PDB	1JR6 1ONB
GB	AAU44826 AAU44828 AAU44832 AAU44836 AAU44841