BMRB Entry 4778

Name: Backbone 1HN, 15N and 13C shifts for GMPPNP-loaded Cdc42 from Candida albicans
Published: 2004-02-13

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PDB ID: 1fi6
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4778
MolProbity Validation Chart

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1HN, 15N and 13C shifts for GMPPNP-loaded Cdc42 from Candida albicans

Deposition date:

2000-07-07

Original release date:

2004-02-13

Authors:

Osborne, Michael; Stevens, Willem; Ping, Xu; Ni, Feng

Citation:

Citation: Osborne, Michael; Su, Z.; Sridaran, V.; Ni, Feng. "Efficient expression of isotopically labeled peptides for high resolution NMR studies: application to the Cdc42/Rac binding domains of virulent kinases in Candida albicans " J. Biomol. NMR 26, 317-326 (2003).
PubMed: 12815258

Assembly members:

Assembly members:
Cell division control protein 42, polymer, 181 residues, Formula weight is not available
5p-guanosyl-imido-triphosphate, non-polymer, 522.196 Da.

Natural source:

Natural source: Common Name: Yeast Taxonomy ID: 5476 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Candida albicans

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cell division control protein 42: GSHMQTIKCVVVGDGAVGKT CLLISYTTSKFPADYVPTVF DNYAVTVMIGDEPFTLGLFD TAGQEDYDRLRPLSYPSTDV FLVCFSVISPASFENVKEKW FPEVHHHCPGVPIIIVGTQT DLRNDDVILQRLHRQKLSPI TQEQGEKLAKELRAVKYVEC SALTQRGLKTVFDEAIVAAL E

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	140
13C chemical shifts	253
15N chemical shifts	140

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Cdc42	1
2	GNP	2

Entities:

Entity 1, Cdc42 181 residues - Formula weight is not available

1

GLY

SER

HIS

MET

GLN

THR

ILE

LYS

CYS

VAL

2

VAL

GLY

ASP

GLY

ALA

VAL

GLY

LYS

THR

3

CYS

LEU

ILE

SER

TYR

THR

SER

LYS

4

PHE

PRO

ALA

ASP

TYR

VAL

PRO

THR

VAL

PHE

5

ASP

ASN

TYR

ALA

VAL

THR

VAL

MET

ILE

GLY

6

ASP

GLU

PRO

PHE

THR

LEU

GLY

LEU

PHE

ASP

7

THR

ALA

GLY

GLN

GLU

ASP

TYR

ASP

ARG

LEU

8

ARG

PRO

LEU

SER

TYR

PRO

SER

THR

ASP

VAL

9

PHE

LEU

VAL

CYS

PHE

SER

VAL

ILE

SER

PRO

10

ALA

SER

PHE

GLU

ASN

VAL

LYS

GLU

LYS

TRP

11

PHE

PRO

GLU

VAL

HIS

CYS

PRO

GLY

12

VAL

PRO

ILE

VAL

GLY

THR

GLN

THR

13

ASP

LEU

ARG

ASN

ASP

VAL

ILE

LEU

GLN

14

ARG

LEU

HIS

ARG

GLN

LYS

LEU

SER

PRO

ILE

15

THR

GLN

GLU

GLN

GLY

GLU

LYS

LEU

ALA

LYS

16

GLU

LEU

ARG

ALA

VAL

LYS

TYR

VAL

GLU

CYS

17

SER

ALA

LEU

THR

GLN

ARG

GLY

LEU

LYS

THR

18

VAL

PHE

ASP

GLU

ALA

ILE

VAL

ALA

LEU

19

GLU

Entity 2, GNP 1 residues - C10 H17 N6 O13 P3 - 522.196 Da.

1

GNP

Samples:

sample_1: Cell division control protein 42, [U-15N; U-13C], 0.7 mM; 5p-guanosyl-imido-triphosphate 7 mM; sodium phosphate 50 mM; magnesium chloride 2 mM; sodium chloride 50 mM

cond_1: pH*: 6.8; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	not available	not available	not available
HN(CO)CA	not available	not available	not available
CBCA(CO)NH	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Bruker DRX 800 MHz

BMRB	4770
EMBL	CAX45095 CCE43086 CCG21049
GB	AAB69764 EAK92670 EAK92699 EDK42478 EEQ42372
REF	XP_001528136 XP_002417442 XP_002548861 XP_003866489 XP_007376934
SP	C4YDI6 P0CY33
AlphaFold	C4YDI6 P0CY33