BMRB Entry 4701

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PDB ID: 1d9s
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4701
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Tumor suppressor INK4: refinement of p16/INK4A structure and determination of p15/INK4B structure by comparative modeling and NMR data

Deposition date:

2000-03-27

Original release date:

2000-05-09

Authors:

Yuan, Chunhua; Selby, Thomas; Byeon, In-Ja; Tsai, Ming-Daw

Citation:

Citation: Yuan, Chunhua; Selby, Thomas; Li, Junan; Byeon, In-Ja; Tsai, Ming-Daw. "Tumor suppressor INK4: refinement of p16/INK4A structure and determination of p15/INK4B structure by comparative modeling and NMR data" Protein Sci. ., .-. (2000).

Assembly members:

Assembly members:
p15, polymer, 136 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mus musculus Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
p15: GSPGIHMLGGSSDAGLATAA ARGQVETVRQLLEAGADPNA LNRFGRRPIQVMMMGSAQVA ELLLLHGAEPNCADPATLTR PVHDAAREGFLDTLVVLHRA GARLDVCDAWGRLPVDLAEE QGHRDIARYLHAATGD

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
1H chemical shifts	745
13C chemical shifts	260
15N chemical shifts	120

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	p15	1

Entities:

Entity 1, p15 136 residues - Formula weight is not available

1

GLY

SER

PRO

GLY

ILE

HIS

MET

LEU

GLY

2

SER

ASP

ALA

GLY

LEU

ALA

THR

ALA

3

ALA

ARG

GLY

GLN

VAL

GLU

THR

VAL

ARG

GLN

4

LEU

GLU

ALA

GLY

ALA

ASP

PRO

ASN

ALA

5

LEU

ASN

ARG

PHE

GLY

ARG

PRO

ILE

GLN

6

VAL

MET

GLY

SER

ALA

GLN

VAL

ALA

7

GLU

LEU

HIS

GLY

ALA

GLU

PRO

8

ASN

CYS

ALA

ASP

PRO

ALA

THR

LEU

THR

ARG

9

PRO

VAL

HIS

ASP

ALA

ARG

GLU

GLY

PHE

10

LEU

ASP

THR

LEU

VAL

LEU

HIS

ARG

ALA

11

GLY

ALA

ARG

LEU

ASP

VAL

CYS

ASP

ALA

TRP

12

GLY

ARG

LEU

PRO

VAL

ASP

LEU

ALA

GLU

13

GLN

GLY

HIS

ARG

ASP

ILE

ALA

ARG

TYR

LEU

14

HIS

ALA

THR

GLY

ASP

Samples:

sample_1: p15, [U-15N], 0.2 – 0.4 mM; HEPES 4 mM; DTT 1 mM; EDTA 5. uM; D2O 5%; H2O 95%

sample_2: p15, [U-15N; U-13C], 0.2 – 0.4 mM

sample_3: p15, [U-15N; U-13C], 0.2 – 0.4 mM

Ex-cond_1: pH: 7.5; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	not available	not available	not available
CBCA(CO)NH	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
1H-15N NOESY	not available	not available	not available
1H-13C NOESY	not available	not available	not available

Software:

XWINNMR v2.0 - Data collection and data procssing

NMR spectrometers:

Bruker DRX 800 MHz
Bruker DMX 600 MHz

PDB	1D9S
DBJ	BAE25719
GB	AAB35360 AAB39833 AAB94534 AAC14569 AAD00232
PIR	I78845
REF	NP_031696 NP_570825 XP_006974062
SP	P55271 P55272
AlphaFold	P55271 P55272