BMRB Entry 4663
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4663
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Lazar, G.; Johnson, E.; Desjarlais, J.; Handel, T.. "Rotamer Strain as a Determinant of Protein Structural Specificity" Protein Sci. 8, 2598-2610 (1999).
PubMed: 10631975
Assembly members:
PROTEIN (1D8 UBIQUITIN), polymer, 76 residues, Formula weight is not available
Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
PROTEIN (1D8 UBIQUITIN): MQLFVKTLTGKTLTVELEPS
DTVENLKAKIQDKEGIPPDQ
QRLIFAGKQLEDGRTLSDYN
LQKESTIHLVLRLRGG
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 273 |
| 15N chemical shifts | 84 |
| 1H chemical shifts | 571 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 1D8-UBIQUITIN | 1 |
Entities:
Entity 1, 1D8-UBIQUITIN 76 residues - Formula weight is not available
| 1 | MET | GLN | LEU | PHE | VAL | LYS | THR | LEU | THR | GLY | ||||
| 2 | LYS | THR | LEU | THR | VAL | GLU | LEU | GLU | PRO | SER | ||||
| 3 | ASP | THR | VAL | GLU | ASN | LEU | LYS | ALA | LYS | ILE | ||||
| 4 | GLN | ASP | LYS | GLU | GLY | ILE | PRO | PRO | ASP | GLN | ||||
| 5 | GLN | ARG | LEU | ILE | PHE | ALA | GLY | LYS | GLN | LEU | ||||
| 6 | GLU | ASP | GLY | ARG | THR | LEU | SER | ASP | TYR | ASN | ||||
| 7 | LEU | GLN | LYS | GLU | SER | THR | ILE | HIS | LEU | VAL | ||||
| 8 | LEU | ARG | LEU | ARG | GLY | GLY |
Samples:
sample_one: PROTEIN (1D8 UBIQUITIN), [U-13C; U-15N], 2 mM; sodium acetate D3 25 mM; sodium azide 0.02%
sample_conditions: ionic strength: 50 mM; pH: 5.80; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D 15N NOESY | sample_one | not available | sample_conditions |
| 3D 13C NOESY | sample_one | not available | sample_conditions |
| 4D 13C/13C NOESY | sample_one | not available | sample_conditions |
| 2D JCCOC | sample_one | not available | sample_conditions |
| 2D JCNC | sample_one | not available | sample_conditions |
| 3D LRCC | sample_one | not available | sample_conditions |
| 3D HACAHB AND HNHA | sample_one | not available | sample_conditions |
| HNHB | sample_one | not available | sample_conditions |
| H/D EXCHANGE FOR H-BONDS | sample_one | not available | sample_conditions |
Software:
No software information available
NMR spectrometers:
- BRUKER DMX 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks