BMRB Entry 4620

Name: Human prion protein variant R220K
Published: 2000-12-06

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PDB ID: 1e1u
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4620
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Human prion protein variant R220K

Deposition date:

2000-05-11

Original release date:

2000-12-06

Authors:

Calzonai, L.; Lysek, D.; Guntert, P.; Von Schroetter, C.; Zahn, R.; Riek, R.; Wuthrich, K.

Citation:

Citation: Calzonai, L.; Lysek, D.; Guntert, P.; Von Schroetter, C.; Zahn, R.; Riek, R.; Wuthrich, K.. "NMR structures of three single-residue variants of the human prion protein" Proc. Natl. Acad. Sci. U. S. A. 97, 8340-8345 (2000).

Assembly members:

Assembly members:
prion protein, polymer, 112 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
prion protein: GSVVGGLGGYMLGSAMSRPI IHFGSDYEDRYYRENMHRYP NQVYYRPMDEYSNQNNFVHD CVNITIKQHTVTTTTKGENF TETDVKMMERVVEQMCITQY EKESQAYYQRGS

Data sets:

assigned_chemical_shifts
- chem_shift_set_1

Data type	Count
13C chemical shifts	355
15N chemical shifts	125
1H chemical shifts	768

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	prion protein	1

Entities:

Entity 1, prion protein 112 residues - Formula weight is not available

1

GLY

SER

VAL

GLY

LEU

GLY

TYR

2

MET

LEU

GLY

SER

ALA

MET

SER

ARG

PRO

ILE

3

ILE

HIS

PHE

GLY

SER

ASP

TYR

GLU

ASP

ARG

4

TYR

ARG

GLU

ASN

MET

HIS

ARG

TYR

PRO

5

ASN

GLN

VAL

TYR

ARG

PRO

MET

ASP

GLU

6

TYR

SER

ASN

GLN

ASN

PHE

VAL

HIS

ASP

7

CYS

VAL

ASN

ILE

THR

ILE

LYS

GLN

HIS

THR

8

VAL

THR

LYS

GLY

GLU

ASN

PHE

9

THR

GLU

THR

ASP

VAL

LYS

MET

GLU

ARG

10

VAL

GLU

GLN

MET

CYS

ILE

THR

GLN

TYR

11

GLU

LYS

GLU

SER

GLN

ALA

TYR

GLN

ARG

12

GLY

SER

Samples:

sample_1: prion protein, [U-13C; U-15N], 1.0 mM; sodium acetate 50 mM

sample_cond_1: ionic strength: 50 mM; pH: 4.5; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	sample_1	not available	sample_cond_1
HN(CO)CA	sample_1	not available	sample_cond_1
HCCH-TOCSY	sample_1	not available	sample_cond_1
15N-resolved (1H,1H)NOESY	sample_1	not available	sample_cond_1
13C-resolved (1H,1H)NOESY	sample_1	not available	sample_cond_1

Software:

No software information available

NMR spectrometers:

Bruker DRX 750 MHz

BMRB	4379 4402 4434 4641 4736 5713
PDB	1FKC 1FO7 1I4M 1QLX 1QLZ 1QM0 1QM1 1QM2 1QM3
DBJ	BAA00011 BAG32276 BAG35206 BAG52189 BAG56832
EMBL	CAA58442 CAB75503 CAM27320
GenBank	AAA19664 AAA60182 AAA68633 AAB59442 AAB59443
REF	NP_000302 NP_001073590 NP_001073591 NP_001073592 NP_898902
SWISS-PROT	P04156 P40252