BMRB Entry 4603

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4603
MolProbity Validation Chart

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Title:
Tertiary structure of apo-D-alanyl carrier protein
Deposition date:
2000-05-05
Original release date:
2001-07-30
Authors:
Volkman, B.; Zhang, Q.; Debabov, D.; Rivera, E.; Kresheck, G.; Neuhaus, F.
Citation:

Citation: Volkman, B.; Zhang, Q.; Debabov, D.; Rivera, E.; Kresheck, G.; Neuhaus, F.. "Biosynthesis of D-alanyl-lipoteichoic acid: the tertiary structure of apo-D-alanyl carrier protein"  Biochemistry 40, 7964-7972 (2001).

Assembly members:

Assembly members:
APO-D-ALANYL CARRIER PROTEIN, polymer, 80 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Lactobacillus casei   Taxonomy ID: 1582   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Lactobacillus casei

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: PDCP1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
APO-D-ALANYL CARRIER PROTEIN: ADEAIKNGVLDILADLTGSD DVKKNLDLNLFETGLLDSMG TVQLLLELQSQFGVDAPVSE FDRKEWDTPNKIIAKVEQAQ

Data sets:
Data typeCount
1H chemical shifts584
13C chemical shifts349
15N chemical shifts88

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1apo-D-alanyl carrier protein1

Entities:

Entity 1, apo-D-alanyl carrier protein 80 residues - Formula weight is not available

1   ALAASPGLUALAILELYSASNGLYVALLEU
2   ASPILELEUALAASPLEUTHRGLYSERASP
3   ASPVALLYSLYSASNLEUASPLEUASNLEU
4   PHEGLUTHRGLYLEULEUASPSERMETGLY
5   THRVALGLNLEULEULEUGLULEUGLNSER
6   GLNPHEGLYVALASPALAPROVALSERGLU
7   PHEASPARGLYSGLUTRPASPTHRPROASN
8   LYSILEILEALALYSVALGLUGLNALAGLN

Samples:

sample_1: APO-D-ALANYL CARRIER PROTEIN, [U-15N], 1.2 mM; phosphate 50 mM; H2O 90%; D2O 10%

sample_2: APO-D-ALANYL CARRIER PROTEIN, [U-15N; U-13C], 1.8 mM; phosphate 50 mM; H2O 90%; D2O 10%

sample_cond_1: pH: 5.8; temperature: 298 K; ionic strength: 50 mM

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablesample_cond_1
3D 13C-separated NOESYnot availablenot availablesample_cond_1
2D 15N-filtered NOESYnot availablenot availablesample_cond_1

Software:

FELIX v95 - data processing

NMRPipe - data processing

DYANA v1.5 - data refinement

XWINNMR v2.3 - data collection

XEASY v1.3 - data analysis

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

PDB
DBJ BAI41283
EMBL CAR86674 CAR89613 CDN21838
GB AAB17659 AAF09203 ABI85297 AER63614 AGP70576
REF WP_005685531 WP_047677124
SP P55153
AlphaFold P55153

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks