BMRB Entry 4578

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4578

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Title:
1H, 13C and 15N NMR Assignment of the Hyperstable Quintuple Mutant of Pseudomonas aeruginosa Cytochrome c-551.
Deposition date:
2000-01-28
Original release date:
2000-11-29
Authors:
Hasegawa, Jun; Sambongi, Yoshihiro; Kobayashi, Yuji; Igarashi, Yasuo
Citation:

Citation: Hasegawa, Jun; Uchiyama, Susumu; Yuko, Tanimoto; Masayuki, Mizutani; Yuji, Kobayashi; Yoshihiro, Sambongi; Igarashi, Yasuo. "Selected Mutations in a Mesophilic Cytochrome c Confer the Stability of a Thermophilic Counterpart"  J. Biol. Chem. 275, 37824-37828 (2000).
PubMed: 10918067

Assembly members:

Assembly members:
Psedomonas aeruginosa cytochrome c-551, polymer, 82 residues, Formula weight is not available
HEC, non-polymer, 618.503 Da.

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pKK223-3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Psedomonas aeruginosa cytochrome c-551: EDPEVLAKNKGCMACHAIDT KMVGPAYKDVAAKYAGQAGA EAYLAQRIKNGSQGVWGPIP MPPNAVSDDEAQTLAKWILS QK

Data sets:
Data typeCount
13C chemical shifts351
15N chemical shifts88
1H chemical shifts531

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Cyt c5511
2heme c2

Entities:

Entity 1, Cyt c551 82 residues - Formula weight is not available

1   GLUASPPROGLUVALLEUALALYSASNLYS
2   GLYCYSMETALACYSHISALAILEASPTHR
3   LYSMETVALGLYPROALATYRLYSASPVAL
4   ALAALALYSTYRALAGLYGLNALAGLYALA
5   GLUALATYRLEUALAGLNARGILELYSASN
6   GLYSERGLNGLYVALTRPGLYPROILEPRO
7   METPROPROASNALAVALSERASPASPGLU
8   ALAGLNTHRLEUALALYSTRPILELEUSER
9   GLNLYS

Entity 2, heme c - C34 H34 Fe N4 O4 - 618.503 Da.

1   HEC

Related Database Links:

PDB 1DVV

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks