BMRB Entry 4578

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4578

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N NMR Assignment of the Hyperstable Quintuple Mutant of Pseudomonas aeruginosa Cytochrome c-551.

Deposition date:

2000-01-28

Original release date:

2000-11-29

Authors:

Hasegawa, Jun; Sambongi, Yoshihiro; Kobayashi, Yuji; Igarashi, Yasuo

Citation:

Citation: Hasegawa, Jun; Uchiyama, Susumu; Yuko, Tanimoto; Masayuki, Mizutani; Yuji, Kobayashi; Yoshihiro, Sambongi; Igarashi, Yasuo. "Selected Mutations in a Mesophilic Cytochrome c Confer the Stability of a Thermophilic Counterpart" J. Biol. Chem. 275, 37824-37828 (2000).
PubMed: 10918067

Assembly members:

Assembly members:
Psedomonas aeruginosa cytochrome c-551, polymer, 82 residues, Formula weight is not available
HEC, non-polymer, 618.503 Da.

Natural source:

Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pKK223-3

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Psedomonas aeruginosa cytochrome c-551: EDPEVLAKNKGCMACHAIDT KMVGPAYKDVAAKYAGQAGA EAYLAQRIKNGSQGVWGPIP MPPNAVSDDEAQTLAKWILS QK

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts

Data type	Count
13C chemical shifts	351
15N chemical shifts	88
1H chemical shifts	531

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Cyt c551	1
2	heme c	2

Entities:

Entity 1, Cyt c551 82 residues - Formula weight is not available

1

GLU

ASP

PRO

GLU

VAL

LEU

ALA

LYS

ASN

LYS

2

GLY

CYS

MET

ALA

CYS

HIS

ALA

ILE

ASP

THR

3

LYS

MET

VAL

GLY

PRO

ALA

TYR

LYS

ASP

VAL

4

ALA

LYS

TYR

ALA

GLY

GLN

ALA

GLY

ALA

5

GLU

ALA

TYR

LEU

ALA

GLN

ARG

ILE

LYS

ASN

6

GLY

SER

GLN

GLY

VAL

TRP

GLY

PRO

ILE

PRO

7

MET

PRO

ASN

ALA

VAL

SER

ASP

GLU

8

ALA

GLN

THR

LEU

ALA

LYS

TRP

ILE

LEU

SER

9

GLN

LYS

Entity 2, heme c - C34 H34 Fe N4 O4 - 618.503 Da.

1

HEC

Samples:

sample_1: Psedomonas aeruginosa cytochrome c-551, [U-95% 13C; U-95% 15N], 1.0 mM

Ex-cond_1: pH: 5.0 na; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D NOESY	sample_1	not available	Ex-cond_1
2D DQF-COSY	sample_1	not available	Ex-cond_1
2D 1H-15N HSQC	sample_1	not available	Ex-cond_1
2D 1H-13C CT-HSQC	sample_1	not available	Ex-cond_1
3D 15N edited NOESY-HSQC	sample_1	not available	Ex-cond_1
3D HNHA	sample_1	not available	Ex-cond_1
3D HNHB	sample_1	not available	Ex-cond_1
3D HNCACB	sample_1	not available	Ex-cond_1
3D CBCA(CO)NNH	sample_1	not available	Ex-cond_1
3D (HB)CBCACO(CA)HA	sample_1	not available	Ex-cond_1
3D HNCO	sample_1	not available	Ex-cond_1
3D HCCH-TOCSY	sample_1	not available	Ex-cond_1
3D 13C,15N edited NOESY-HSQC	sample_1	not available	Ex-cond_1

Software:

PIPP -

NMR spectrometers:

Varian INOVA 600 MHz