BMRB Entry 4564

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4564

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
NMR Structure of the Bovine Prion Protein
Deposition date:
1999-12-15
Original release date:
2000-05-24
Authors:
Lopez Garcia, Francisco; Zahn, Ralph; Riek, Roland; Billeter, Martin; Wuthrich, Kurt
Citation:

Citation: Lopez Garcia, Francisco; Zahn, Ralph; Riek, Roland; Billeter, Martin; Wuthrich, Kurt. "NMR Structure of the Bovine Prion Protein"  Proc. Natl. Acad. Sci. U.S.A. ., .-..

Assembly members:

Assembly members:
bovine prion protein, polymer, 264 residues, 13137 Da.

Natural source:

Natural source:   Common Name: bovine   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
bovine prion protein: MVKSHIGSWILVLFVAMWSD VGLSKKRPKPGGGWNTGGSR YPGQGSPGGNRYPPQGGGGW GQPHGGGWGQPHGGGWGQPH GGGWGQPHGGGWGQPHGGGG WGQGGTHGQWNKPSKPKTNM KHVAGAAAAGAVVGGLGGYM LGSAMSRPLIHFGSDYEDRY YRENMHRYPNQVYYRPVDQY SNQNNFVHDCVNITVKEHTV TTTTKGENFTETDIKMMERV VEQMCITQYQRESQAYYQRG ASVILFSSPPVILLISFLIF LIVG

Data sets:
Data typeCount
13C chemical shifts483
15N chemical shifts226
1H chemical shifts1199

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1bPrP(23-230)1

Entities:

Entity 1, bPrP(23-230) 264 residues - 13137 Da.

1   METVALLYSSERHISILEGLYSERTRPILE
2   LEUVALLEUPHEVALALAMETTRPSERASP
3   VALGLYLEUSERLYSLYSARGPROLYSPRO
4   GLYGLYGLYTRPASNTHRGLYGLYSERARG
5   TYRPROGLYGLNGLYSERPROGLYGLYASN
6   ARGTYRPROPROGLNGLYGLYGLYGLYTRP
7   GLYGLNPROHISGLYGLYGLYTRPGLYGLN
8   PROHISGLYGLYGLYTRPGLYGLNPROHIS
9   GLYGLYGLYTRPGLYGLNPROHISGLYGLY
10   GLYTRPGLYGLNPROHISGLYGLYGLYGLY
11   TRPGLYGLNGLYGLYTHRHISGLYGLNTRP
12   ASNLYSPROSERLYSPROLYSTHRASNMET
13   LYSHISVALALAGLYALAALAALAALAGLY
14   ALAVALVALGLYGLYLEUGLYGLYTYRMET
15   LEUGLYSERALAMETSERARGPROLEUILE
16   HISPHEGLYSERASPTYRGLUASPARGTYR
17   TYRARGGLUASNMETHISARGTYRPROASN
18   GLNVALTYRTYRARGPROVALASPGLNTYR
19   SERASNGLNASNASNPHEVALHISASPCYS
20   VALASNILETHRVALLYSGLUHISTHRVAL
21   THRTHRTHRTHRLYSGLYGLUASNPHETHR
22   GLUTHRASPILELYSMETMETGLUARGVAL
23   VALGLUGLNMETCYSILETHRGLNTYRGLN
24   ARGGLUSERGLNALATYRTYRGLNARGGLY
25   ALASERVALILELEUPHESERSERPROPRO
26   VALILELEULEUILESERPHELEUILEPHE
27   LEUILEVALGLY

Samples:

sample_1: bovine prion protein 1.0 mM

sample_2: bovine prion protein, [U-15N], 1.0 mM

sample_3: bovine prion protein, [U-15N; U-13C], 1.0 mM

sample_conditions: pH: 4.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-1H-15N NOESYnot availablenot availablenot available
1H-1H-13C NOESYnot availablenot availablenot available
HCCH COSYnot availablenot availablenot available
HCCH TOCSYnot availablenot availablenot available
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HN(COCA)NNHnot availablenot availablenot available
ct-HNCAnot availablenot availablenot available

Software:

PROSA -

XEASY -

DYANA -

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker DRX 750 MHz

Related Database Links:

PDB
DBJ BAA01467 BAA01468 BAA19253 BAE78583 BAE78586
EMBL CAA39368 CAA52781 CAC37367
GB AAB25514 AAC43030 AAD13290 AAD19984 AAD19998
REF NP_001258555 NP_001291815 NP_851358 XP_005906596 XP_006039785
SP B5SY89 P10279 P40242 Q5UAF1 Q5UJG1
TPG DAA23426
AlphaFold B5SY89 P10279 P40242 Q5UAF1 Q5UJG1

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks