BMRB Entry 4560
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4560
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NMR-STAR v3 text file.
XML gzip file.
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Citation: Hemmi, Hikaru; Chae, Young; Studts, Joey; Fox, Brian; Markley, John. "Letter to the Editor: Assignment of 1H, 13C and 15N signals of a recombinant
effector protein (T4MOD) in Toluene-4-monooxygenase complex" J. Biomol. NMR 16, 359-360 (2000).
Assembly members:
T4MOD, polymer, 102 residues, Formula weight is not available
Natural source: Common Name: Pseudomonas mendocina Taxonomy ID: 300 Superkingdom: Eubacteria Kingdom: not available Genus/species: Pseudomonas mendocina
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
T4MOD: STLADQALHNNNVGPIIRAG
DLVEPVIETAEIDNPGKEIT
VEDRRAYVRIAAEGELILTR
KTLEEQLGRPFNMQELEINL
ASFAGQIQADEDQIRFYFDK
TM
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 730 |
| 13C chemical shifts | 458 |
| 15N chemical shifts | 111 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | T4MOD | 1 |
Entities:
Entity 1, T4MOD 102 residues - Formula weight is not available
| 1 | SER | THR | LEU | ALA | ASP | GLN | ALA | LEU | HIS | ASN | ||||
| 2 | ASN | ASN | VAL | GLY | PRO | ILE | ILE | ARG | ALA | GLY | ||||
| 3 | ASP | LEU | VAL | GLU | PRO | VAL | ILE | GLU | THR | ALA | ||||
| 4 | GLU | ILE | ASP | ASN | PRO | GLY | LYS | GLU | ILE | THR | ||||
| 5 | VAL | GLU | ASP | ARG | ARG | ALA | TYR | VAL | ARG | ILE | ||||
| 6 | ALA | ALA | GLU | GLY | GLU | LEU | ILE | LEU | THR | ARG | ||||
| 7 | LYS | THR | LEU | GLU | GLU | GLN | LEU | GLY | ARG | PRO | ||||
| 8 | PHE | ASN | MET | GLN | GLU | LEU | GLU | ILE | ASN | LEU | ||||
| 9 | ALA | SER | PHE | ALA | GLY | GLN | ILE | GLN | ALA | ASP | ||||
| 10 | GLU | ASP | GLN | ILE | ARG | PHE | TYR | PHE | ASP | LYS | ||||
| 11 | THR | MET |
Samples:
sample_1: T4MOD, [U-13C; U-15N], 1.1 mM
sample_2: T4MOD2.5 – 3.5 mM
cond_1: pH: 7.0; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | not available | not available | not available |
| 3D HNCA | not available | not available | not available |
| 3D HNCO | not available | not available | not available |
| 3D HN(CO)CA | not available | not available | not available |
| 3D CCONH | not available | not available | not available |
| 3D HNCACB | not available | not available | not available |
| 3D HCCONH | not available | not available | not available |
| 3D HCCH-COSY | not available | not available | not available |
| 3D HCCH-TOCSY | not available | not available | not available |
| 2D 1H-1H NOESY | not available | not available | not available |
| 2D 1H-1H TOCSY | not available | not available | not available |
| 3D CT-13C-HSQC | not available | not available | not available |
| 3D 1H-1H-15N NOESY | not available | not available | not available |
| 3D 13C-1H-1H NOESY | not available | not available | not available |
| 3D 15N TOCSY-HSQC | not available | not available | not available |
Software:
FELIX v95 - processing of NMR data
Sparky - assignment
NMR spectrometers:
- Bruker DMX 500 MHz
- Bruker DMX 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks