BMRB Entry 4554
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4554
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Citation: Zaborowski, Eduardo; Chung, John; Kroon, Gerard; Dyson, H.; Wright, Peter. "Letter to the Editor: Backbone HN, N, Ca, C' and Cb assignments of the
19 kDa DHFR/NADPH complex at 9C and pH 7.6" J. Biomol. NMR 16, 349-350 (2000).
Assembly members:
Dihydrofolate reductase, polymer, 159 residues, 18200 Da.
NDP, non-polymer, 745.421 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-22b+
Entity Sequences (FASTA):
Dihydrofolate reductase: MISLIAALAVDRVIGMENAM
PWNLPADLAWFKRNTLNKPV
IMGRHTWESIGRPLPGRKNI
ILSSQPGTDDRVTWVKSVDE
AIAACGDVPEIMVIGGGRVY
EQFLPKAQKLYLTHIDAEVE
GDTHFPDYEPDDWESVFSEF
HDADAQNSHSYCFEILERR
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 147 |
| 13C chemical shifts | 449 |
| 15N chemical shifts | 147 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | DHFR | 1 |
| 2 | NADPH | 2 |
Entities:
Entity 1, DHFR 159 residues - 18200 Da.
| 1 | MET | ILE | SER | LEU | ILE | ALA | ALA | LEU | ALA | VAL | ||||
| 2 | ASP | ARG | VAL | ILE | GLY | MET | GLU | ASN | ALA | MET | ||||
| 3 | PRO | TRP | ASN | LEU | PRO | ALA | ASP | LEU | ALA | TRP | ||||
| 4 | PHE | LYS | ARG | ASN | THR | LEU | ASN | LYS | PRO | VAL | ||||
| 5 | ILE | MET | GLY | ARG | HIS | THR | TRP | GLU | SER | ILE | ||||
| 6 | GLY | ARG | PRO | LEU | PRO | GLY | ARG | LYS | ASN | ILE | ||||
| 7 | ILE | LEU | SER | SER | GLN | PRO | GLY | THR | ASP | ASP | ||||
| 8 | ARG | VAL | THR | TRP | VAL | LYS | SER | VAL | ASP | GLU | ||||
| 9 | ALA | ILE | ALA | ALA | CYS | GLY | ASP | VAL | PRO | GLU | ||||
| 10 | ILE | MET | VAL | ILE | GLY | GLY | GLY | ARG | VAL | TYR | ||||
| 11 | GLU | GLN | PHE | LEU | PRO | LYS | ALA | GLN | LYS | LEU | ||||
| 12 | TYR | LEU | THR | HIS | ILE | ASP | ALA | GLU | VAL | GLU | ||||
| 13 | GLY | ASP | THR | HIS | PHE | PRO | ASP | TYR | GLU | PRO | ||||
| 14 | ASP | ASP | TRP | GLU | SER | VAL | PHE | SER | GLU | PHE | ||||
| 15 | HIS | ASP | ALA | ASP | ALA | GLN | ASN | SER | HIS | SER | ||||
| 16 | TYR | CYS | PHE | GLU | ILE | LEU | GLU | ARG | ARG |
Entity 2, NADPH - C21 H30 N7 O17 P3 - 745.421 Da.
| 1 | NDP |
Related Database Links:
| BMRB | 11492 25019 |
| PDB | 1DDR 1DDS 1DHI 1DHJ 1DRA 1DRB 1DRE 1DRH 1DYH 1DYI 1DYJ 1JOL 1JOM 1RA1 1RA2 1RA3 1RA8 1RA9 1RB2 1RB3 1RC4 1RD7 1RE7 1RF7 1RG7 1RH3 1RX1 1RX2 1RX3 1RX4 1RX5 1RX6 1RX7 1RX8 1RX9 1TDR 2ANO 2ANQ 2DRC 2INQ 3DAU 3DRC 3K74 3KFY 3OCH 3QL0 3QL3 3QYL 3QYO 3R33 4DFR 4EIG 4EIZ 4EJ1 4FHB 4I13 4I1N 4KJJ 4KJK 4KJL 4NX6 4NX7 4P3Q 4P3R 4P66 4P68 4PSS 4PST 4PSY 4PTH 4PTJ 4QLE 4QLF 4QLG 4RGC 5CC9 5CCC 5DFR 6DFR 7DFR |
| DBJ | BAA05974 BAB33474 BAB96616 BAG75573 BAI23410 |
| EMBL | CAA28755 CAD01243 CAP74618 CAQ30568 CAQ87642 |
| GB | AAA87976 AAC73159 AAG54351 AAN41711 AAN78554 |
| PIR | AC0513 |
| REF | NP_308078 NP_414590 NP_454699 NP_706004 WP_000378105 |
| SP | P0ABQ4 P0ABQ5 P0ABQ6 |
| AlphaFold | P0ABQ4 P0ABQ5 P0ABQ6 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks