BMRB Entry 4519

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4519
MolProbity Validation Chart

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Title:
HUMAN TRANSLATION INITIATION FACTOR EIF1A
Deposition date:
1999-10-20
Original release date:
2000-07-12
Authors:
Battiste, J.; Pestova, T.; Hellen, C.; Wagner, G.
Citation:

Citation: Battiste, J.; Pestova, T.; Hellen, C.; Wagner, G.. "The eIF1A solution structure reveals a large RNA-binding surface important for scanning function"  Mol Cell 5, 109-119 (2000).
PubMed: 10678173

Assembly members:

Assembly members:
Translation initiation factor 1A, polymer, 157 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE31

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Translation initiation factor 1A: MRGSHHHHHHTDPMPKNKGK GGKNRRRGKNENESEKRELV FKEDGQEYAQVIKMLGNGRL EAMCFDGVKRLCHIRGKLRK KVWINTSDIILVGLRDYQDN KADVILKYNADEARSLKAYG ELPEHAKINETDTFGPGDDD EIQFDDIGDDDEDIDDI

Data sets:
Data typeCount
1H chemical shifts855
13C chemical shifts515
15N chemical shifts131

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1EIF1A1

Entities:

Entity 1, EIF1A 157 residues - Formula weight is not available

1   METARGGLYSERHISHISHISHISHISHIS
2   THRASPPROMETPROLYSASNLYSGLYLYS
3   GLYGLYLYSASNARGARGARGGLYLYSASN
4   GLUASNGLUSERGLULYSARGGLULEUVAL
5   PHELYSGLUASPGLYGLNGLUTYRALAGLN
6   VALILELYSMETLEUGLYASNGLYARGLEU
7   GLUALAMETCYSPHEASPGLYVALLYSARG
8   LEUCYSHISILEARGGLYLYSLEUARGLYS
9   LYSVALTRPILEASNTHRSERASPILEILE
10   LEUVALGLYLEUARGASPTYRGLNASPASN
11   LYSALAASPVALILELEULYSTYRASNALA
12   ASPGLUALAARGSERLEULYSALATYRGLY
13   GLULEUPROGLUHISALALYSILEASNGLU
14   THRASPTHRPHEGLYPROGLYASPASPASP
15   GLUILEGLNPHEASPASPILEGLYASPASP
16   ASPGLUASPILEASPASPILE

Samples:

sample_1: Translation initiation factor 1A, [U-13C; U-15N], 0.8 mM; phosphate buffer 10 mM; NaCl 500 mM; DTT 1 mM; EDTA 0.1 mM

sample_2: Translation initiation factor 1A, [U-15N], 0.5 mM; phosphate buffer 10 mM; NaCl 500 mM; DTT 1 mM; EDTA 0.1 mM

sample_3: Translation initiation factor 1A 1.2 mM; phosphate buffer 10 mM; NaCl 500 mM; DTT 1 mM; EDTA 0.1 mM

sample_4: Translation initiation factor 1A, [U-15N]-Lys, 0.4 mM; phosphate buffer 10 mM; NaCl 500 mM; DTT 1 mM; EDTA 0.1 mM

sample_cond_1: pH: 7.5; temperature: 298 K; ionic strength: 500 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available

Software:

VNMR v6.1 - Collection

FELIX v98 - Processing

XEASY v1.2 - Data analysis

X-PLOR v3.851 - Structure solution

TALOS v1.0 - Data analysis

NMR spectrometers:

  • Varian Unity 500 MHz

Related Database Links:

PDB
DBJ BAB23869 BAB24942 BAB26034 BAB28110 BAB28428
EMBL CAG32501 CAH91368 CAJ18543 CAJ82618 CDQ69064
GB AAA19812 AAC51834 AAC63934 AAH00793 AAH05248
REF NP_001004814 NP_001008773 NP_001008977 NP_001020500 NP_001020624
SP O14602 P47813 P47814 Q5RA42 Q60872
TPG DAA12633
AlphaFold O14602 P47813 P47814 Q5RA42 Q60872

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks