BMRB Entry 4460

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4460

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Title:
1H, 15N and 13C Resonance Assignments for the C-terminal Protein Interaction Region of the 32 kDa Subunit of Human Replication Protein A
Deposition date:
1999-11-16
Original release date:
2005-04-25
Authors:
Mer, Georges; Edwards, Aled; Chazin, Walter
Citation:

Citation: Mer, Georges; Edwards, Aled; Chazin, Walter. "1H, 15N and 13C Resonance Assignments for the C-terminal Protein Interaction Region of the 32 kDa Subunit of Human Replication Protein A"  J. Biomol. NMR 17, 179-180 (2000).

Assembly members:

Assembly members:
Replication Protein A, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Replication Protein A: GSHMANSQPSAGRAPISNPG MSEAGNFGGNSFMPANGLTV AQNQVLNLIKACPRPEGLNF QDLKNQLKHMSVSSIKQAVD FLSNEGHIYSTVDDDHFKST DAE

Data sets:
Data typeCount
1H chemical shifts631
13C chemical shifts277
15N chemical shifts104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RPA321

Entities:

Entity 1, RPA32 103 residues - Formula weight is not available

1   GLYSERHISMETALAASNSERGLNPROSER
2   ALAGLYARGALAPROILESERASNPROGLY
3   METSERGLUALAGLYASNPHEGLYGLYASN
4   SERPHEMETPROALAASNGLYLEUTHRVAL
5   ALAGLNASNGLNVALLEUASNLEUILELYS
6   ALACYSPROARGPROGLUGLYLEUASNPHE
7   GLNASPLEULYSASNGLNLEULYSHISMET
8   SERVALSERSERILELYSGLNALAVALASP
9   PHELEUSERASNGLUGLYHISILETYRSER
10   THRVALASPASPASPHISPHELYSSERTHR
11   ASPALAGLU

Samples:

sample_1: Replication Protein A, [U-13C; U-15N], 1 mM; phosphate buffer 25 mM; DTT 5 mM; H2O 93%; D2O 7%

sample_2: Replication Protein A, [U-10% 13C; U-15N], 1 mM; phosphate buffer 25 mM; DTT 5 mM; H2O 93%; D2O 7%

sample_3: Replication Protein A, [U-15N], 1 mM; phosphate buffer 25 mM; DTT 5 mM; H2O 93%; D2O 7%

sample_4: Replication Protein A, NA, 1 mM; phosphate buffer 25 mM; DTT 5 mM; H2O 93%; D2O 7%

sample_conditions_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availablenot available
2D 1H-13C HSQCnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
2D DQF-COSYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
2D 2Qnot availablenot availablenot available
3D HNCACBnot availablenot availablenot available
3D CBCA(CO)NHnot availablenot availablenot available
3D C(CO)NH-TOCSYnot availablenot availablenot available
3D H(CCO)NH-TOCSYnot availablenot availablenot available
3D HBHA(CBCACO)NHnot availablenot availablenot available
3D HNHAnot availablenot availablenot available
3D HNHBnot availablenot availablenot available
3D HACAHB-COSYnot availablenot availablenot available
3D 15N edited TOCSYnot availablenot availablenot available
3D 15N edited NOESYnot availablenot availablenot available
3D 13C edited NOESYnot availablenot availablenot available

Software:

FELIX v97, MSI - spectra Processing, spectra Analysis

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker AMX 600 MHz
  • Bruker DRX 600 MHz
  • Bruker DMX 750 MHz
  • Bruker DRX 800 MHz

Related Database Links:

BMRB 17060
PDB
DBJ BAG35795 BAG59456 BAG60901 BAG62374 BAI47325
EMBL CAG29344 CAH90667
GB AAA36560 AAH01630 AAH12157 AAH21257 AAX84514
REF NP_001125362 NP_001247563 NP_001273005 NP_001284487 NP_002937
SP P15927 Q5RC43
AlphaFold P15927 Q5RC43

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks