BMRB Entry 4434

Name: NMR Solution Structure of the Human Prion Protein
Published: 2000-02-01

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4434

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Solution Structure of the Human Prion Protein

Deposition date:

1999-10-06

Original release date:

2000-02-01

Authors:

Zahn, Ralph; Aizhuo, Liu; Luhrs, Thorsten; Calzolai, Luigi; von Schroetter, Christine; Lopez Garcia, Francisco; Riek, Roland; Wider, Gerhard; Billeter, Martin; Wuthrich, Kurt

Citation:

Citation: Zahn, Ralph; Liu, Aizhuo; Luhrs, Thorsten; Riek, Roland; von Schroetter, Christine; Lopez Garcia, Francisco; Billeter, Martin; Calzolai, Luigi; Wider, Gerhard; Wuthrich, Kurt. "NMR Solution Structure of the Human Prion Protein" Proc. Natl. Acad. Sci. U. S. A. 97, 145-150 (2000).

Assembly members:

Assembly members:
human prion protein, polymer, 143 residues, 16061 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: metazoa Genus/species: homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human prion protein: GSGQGGGTHSQWNKPSKPKT NMKHMAGAAAAGAVVGGLGG YMLGSAMSRPIIHFGSDYED RYYRENMHRYPNQVYYRPMD EYSNQNNFVHDCVNITIKQH TVTTTTKGENFTETDVKMME RVVEQMCITQYERESQAYYQ RGS

Data sets:

assigned_chemical_shifts
- CSS

Data type	Count
1H chemical shifts	954
13C chemical shifts	441
15N chemical shifts	160

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hPrP(90-230)	1

Entities:

Entity 1, hPrP(90-230) 143 residues - 16061 Da.

1

GLY

SER

GLY

GLN

GLY

THR

HIS

SER

2

GLN

TRP

ASN

LYS

PRO

SER

LYS

PRO

LYS

THR

3

ASN

MET

LYS

HIS

MET

ALA

GLY

ALA

4

ALA

GLY

ALA

VAL

GLY

LEU

GLY

5

TYR

MET

LEU

GLY

SER

ALA

MET

SER

ARG

PRO

6

ILE

HIS

PHE

GLY

SER

ASP

TYR

GLU

ASP

7

ARG

TYR

ARG

GLU

ASN

MET

HIS

ARG

TYR

8

PRO

ASN

GLN

VAL

TYR

ARG

PRO

MET

ASP

9

GLU

TYR

SER

ASN

GLN

ASN

PHE

VAL

HIS

10

ASP

CYS

VAL

ASN

ILE

THR

ILE

LYS

GLN

HIS

11

THR

VAL

THR

LYS

GLY

GLU

ASN

12

PHE

THR

GLU

THR

ASP

VAL

LYS

MET

GLU

13

ARG

VAL

GLU

GLN

MET

CYS

ILE

THR

GLN

14

TYR

GLU

ARG

GLU

SER

GLN

ALA

TYR

GLN

15

ARG

GLY

SER

Samples:

sample_1: human prion protein 2.0 mM

sample_2: human prion protein, [U-15N], 2.0 mM

sample_3: human prion protein, [U-15N; U-13C], 2.0 mM

Ex-cond_1: pH: 4.5 N/A; temperature: 293 K; ionic strength: 0.01 M

Experiments:

Name	Sample	Sample state	Sample conditions
1H-1H-15N NOESY	not available	not available	not available
1H-1H-13C NOESY	not available	not available	not available
HCCH COSY	not available	not available	not available
HCCH TOCSY	not available	not available	not available
HNCA	not available	not available	not available
HNCACB	not available	not available	not available

Software:

PROSA -

XEASY -

DYANA -

NMR spectrometers:

Bruker DRX 600 MHz
Bruker DRX 750 MHz

BMRB	15676 16743 16757 17714 17756 17757 17780 18426 18550 19268 4379 4402 4620 4641 4736
PDB	1E1G 1E1J 1E1P 1E1S 1E1U 1E1W 1FKC 1FO7 1H0L 1HJM 1HJN 1I4M 1QLX 1QLZ 1QM0 1QM1 1QM2 1QM3 2K1D 2KUN 2LEJ 2LFT 2LSB 2LV1 2M8T 2W9E 3HAF 3HAK 3HEQ 3HER 3HES 3HJ5 3HJX 4DGI 4KML 4N9O
DBJ	BAA00011 BAF62360 BAG32276 BAG32277 BAG32278
EMBL	CAA58442 CAG46836 CAG46869 CAH92912
GB	AAA19664 AAA60182 AAA68632 AAA68633 AAB59442
REF	NP_000302 NP_001009093 NP_001073590 NP_001073591 NP_001073592
SP	P04156 P40252 P40256 P61766 P61767
TPE	CAJ43808
AlphaFold	P04156 P40252 P40256 P61766 P61767