BMRB Entry 4426

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PDB ID: 2bdo
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4426
MolProbity Validation Chart

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Title:
Solution Structure of Holo-biotinyl Domain from Acetyl Coenzyme A Carboxylase of Escherichia coli Determined by Triple-Resonance NMR Spectroscopy
Deposition date:
1999-09-29
Original release date:
2000-06-12
Authors:
Roberts, E.; Shu, N.; Howard, M.; Broadhurst, R.; Chapman-smith, A.; Wallace, J.; Morris, T.; Cronan, J.; Perham, R.
Citation:

Citation: Roberts, E.; Shu, N.; Howard, M.; Broadhurst, R.; Chapman-smith, A.; Wallace, J.; Morris, T.; Cronan, J.; Perham, R.. "Solution Structure of Apo- and Holo-biotinyl Domain from Acetyl Coenzyme A Carboxylase of Escherichia coli Determined by Triple-Resonance NMR Spectroscopy"  Biochemistry 38, 5045-5053 (1999).

Assembly members:

Assembly members:
ACETYL-COA CARBOXYLASE, polymer, 82 residues, Formula weight is not available
BIOTIN, non-polymer, 244.311 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ACETYL-COA CARBOXYLASE: AAEISGHIVRSPMVGTFYRT PSPDAKAFIEVGQKVNVGDT LCIVEAMKMMNQIEADKSGT VKAILVESGQPVEFDEPLVV IE

Data sets:
Data typeCount
13C chemical shifts269
15N chemical shifts83
1H chemical shifts531

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1acc biotinyl domain1
2btn2

Entities:

Entity 1, acc biotinyl domain 82 residues - Formula weight is not available

1   ALAALAGLUILESERGLYHISILEVALARG
2   SERPROMETVALGLYTHRPHETYRARGTHR
3   PROSERPROASPALALYSALAPHEILEGLU
4   VALGLYGLNLYSVALASNVALGLYASPTHR
5   LEUCYSILEVALGLUALAMETLYSMETMET
6   ASNGLNILEGLUALAASPLYSSERGLYTHR
7   VALLYSALAILELEUVALGLUSERGLYGLN
8   PROVALGLUPHEASPGLUPROLEUVALVAL
9   ILEGLU

Entity 2, btn - C10 H16 N2 O3 S - 244.311 Da.

1   BTN

Related Database Links:

BMRB 4425
PDB 1A6X 1BDO 2BDO 3BDO
DBJ BAB37550 BAE77296
EMBL CAA32933 CAD07894
GenBank AAA23408 AAA23744 AAA23745 AAA58058 AAC76287
PIR AH0912
REF AP_003795 NP_289823 NP_312154 NP_417721 NP_457755
SWISS-PROT P0ABD8 P0ABD9 P0ABE0 P0ABE1

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