BMRB Entry 4410
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4410
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Citation: Lu, Shanyun; Liang, Songping; Gu, Xiaocheng. "Three dimensional structure of Selenocosmia huwena Lectin-I (SHL-I) from the
venom of the spider Selenocosmia huwena by 2D-NMR" J. Protein Chem. 18, 609-617 (1999).
Assembly members:
Selenocosmia huwena lectin-I, polymer, 32 residues, Formula weight is not available
Natural source: Common Name: Chinese bird spider Taxonomy ID: 29017 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Selenocosmia huwena
Experimental source: Production method: natural source
Entity Sequences (FASTA):
Selenocosmia huwena lectin-I: GCLGDKCDYNNGCCSGYVCS
RTWKWCVLAGPW
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 1H chemical shifts | 197 |
| coupling constants | 11 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | SHL-I | 1 |
Entities:
Entity 1, SHL-I 32 residues - Formula weight is not available
| 1 | GLY | CYS | LEU | GLY | ASP | LYS | CYS | ASP | TYR | ASN | ||||
| 2 | ASN | GLY | CYS | CYS | SER | GLY | TYR | VAL | CYS | SER | ||||
| 3 | ARG | THR | TRP | LYS | TRP | CYS | VAL | LEU | ALA | GLY | ||||
| 4 | PRO | TRP |
Samples:
sample_1: Selenocosmia huwena lectin-I 5.0 mM
Ex-cond_1: pH: 4.5; temperature: 283 K
Ex-cond_2: pH*: 4.5; temperature: 292 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H COSY | not available | not available | not available |
| 2D 1H-1H DQF-COSY | not available | not available | not available |
| 2D 1H-1H NOESY | not available | not available | not available |
| 2D 1H-1H TOCSY | not available | not available | not available |
Software:
SYBYL v6.3 -
NMR spectrometers:
- Varian UnityPlus 600 MHz
- Bruker DMX 500 MHz