BMRB Entry 4407

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PDB ID: 1cmz
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4407
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR chemical shift assignment of human GAIP (Galpha Interacting Protein): A regulator of G protein signaling

Deposition date:

1999-09-15

Original release date:

1999-11-11

Authors:

de Alba, E.; De Vries, L.; Farquhar, M.; Tjandra, N.

Citation:

Citation: de Alba, E.; De Vries, L.; Farquhar, M.; Tjandra, N.. "Solution structure of human GAIP (G alpha interacting protein). A regulator of G protein signaling" J. Mol. Biol. 291, 927-939 (1999).

Assembly members:

Assembly members:
Gaip protein, polymer, 153 residues, 17321 Da.

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Gaip protein: GSPGISGGGGGIPSPEEVQS WAQSFDKLMHSPAGRSVFRA FLRTEYSEENMLFWLACEEL KAEANQHVVDEKARLIYEDY VSILSPKEVSLDSRVREGIN KKMQEPSAHTFDDAQLQIYT LMHRDSYPRFLSSPTYRALL PWVDSSSSLIHRD

Data sets:

assigned_chemical_shifts
- chem_shift

Data type	Count
13C chemical shifts	452
15N chemical shifts	125
1H chemical shifts	899

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	gaip	1

Entities:

Entity 1, gaip 153 residues - 17321 Da.

1

GLY

SER

PRO

GLY

ILE

SER

GLY

2

GLY

ILE

PRO

SER

PRO

GLU

VAL

GLN

SER

3

TRP

ALA

GLN

SER

PHE

ASP

LYS

LEU

MET

HIS

4

SER

PRO

ALA

GLY

ARG

SER

VAL

PHE

ARG

ALA

5

PHE

LEU

ARG

THR

GLU

TYR

SER

GLU

ASN

6

MET

LEU

PHE

TRP

LEU

ALA

CYS

GLU

LEU

7

LYS

ALA

GLU

ALA

ASN

GLN

HIS

VAL

ASP

8

GLU

LYS

ALA

ARG

LEU

ILE

TYR

GLU

ASP

TYR

9

VAL

SER

ILE

LEU

SER

PRO

LYS

GLU

VAL

SER

10

LEU

ASP

SER

ARG

VAL

ARG

GLU

GLY

ILE

ASN

11

LYS

MET

GLN

GLU

PRO

SER

ALA

HIS

THR

12

PHE

ASP

ALA

GLN

LEU

GLN

ILE

TYR

THR

13

LEU

MET

HIS

ARG

ASP

SER

TYR

PRO

ARG

PHE

14

LEU

SER

PRO

THR

TYR

ARG

ALA

LEU

15

PRO

TRP

VAL

ASP

SER

LEU

ILE

16

HIS

ARG

ASP

Samples:

sample_1: Gaip protein, [U-13C; U-15N], 0.6 – 1.0 mM

sample_cond_1: ionic strength: 5 mM; pH: 7.2; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
CBCACONH	not available	not available	not available
CBCANH	not available	not available	not available
HBHACONH	not available	not available	not available
HNCA	not available	not available	not available
HCCH-TOCSY	not available	not available	not available
C-CNOESY	not available	not available	not available
N-CNOESY	not available	not available	not available
H-HNOESY	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Bruker DMX 600 MHz

PDB	1CMZ
EMBL	CAG33711
GB	AAI23822 EDL07441 EDL07445 EPY87810
REF	NP_001070383 XP_003462829 XP_003787844 XP_005214874 XP_006045501
SP	Q08DC7
TPG	DAA23284
AlphaFold	Q08DC7