BMRB Entry 4402

Name: 1H, 13C and 15N chemical shift assignment of human prion protein hPrP(23-230)
Published: 2000-02-01

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4402

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N chemical shift assignment of human prion protein hPrP(23-230)

Deposition date:

1999-09-14

Original release date:

2000-02-01

Authors:

Liu, Aizhuo; Riek, Roland; Wider, Gerhard; von Schroetter, Christine; Zahn, Ralph; Wuthrich, Kurt

Citation:

Citation: Zahn, Ralph; Liu, Aizhuo; Luhrs, Thorsten; Riek, Roland; von Schroetter, Christine; Lopez Garcia, Francisco; Billeter, Martin; Calzolai, Luigi; Wider, Gerhard; Wuthrich, Kurt. "NMR Solution Structure of the Human Prion Protein." Proc. Natl. Acad. Sci. U. S. A. 97, 145-150 (2000).

Assembly members:

Assembly members:
human prion protein, polymer, 210 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: metazoa Genus/species: homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
human prion protein: GSKKRPKPGGWNTGGSRYPG QGSPGGNRYPPQGGGGWGQP HGGGWGQPHGGGWGQPHGGG WGQPHGGGWGQGGGTHSQWN KPSKPKTNMKHMAGAAAAGA VVGGLGGYMLGSAMSRPIIH FGSDYEDRYYRENMHRYPNQ VYYRPMDEYSNQNNFVHDCV NITIKQHTVTTTTKGENFTE TDVKMMERVVEQMCITQYER ESQAYYQRGS

Data sets:

assigned_chemical_shifts
- shift_set_1

Data type	Count
13C chemical shifts	600
15N chemical shifts	246
1H chemical shifts	1311

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hPrP(23-230)	1

Entities:

Entity 1, hPrP(23-230) 210 residues - Formula weight is not available

1	GLY	SER	LYS	LYS	ARG	PRO	LYS	PRO	GLY	GLY
2	TRP	ASN	THR	GLY	GLY	SER	ARG	TYR	PRO	GLY
3	GLN	GLY	SER	PRO	GLY	GLY	ASN	ARG	TYR	PRO
4	PRO	GLN	GLY	GLY	GLY	GLY	TRP	GLY	GLN	PRO
5	HIS	GLY	GLY	GLY	TRP	GLY	GLN	PRO	HIS	GLY
6	GLY	GLY	TRP	GLY	GLN	PRO	HIS	GLY	GLY	GLY
7	TRP	GLY	GLN	PRO	HIS	GLY	GLY	GLY	TRP	GLY
8	GLN	GLY	GLY	GLY	THR	HIS	SER	GLN	TRP	ASN
9	LYS	PRO	SER	LYS	PRO	LYS	THR	ASN	MET	LYS
10	HIS	MET	ALA	GLY	ALA	ALA	ALA	ALA	GLY	ALA
11	VAL	VAL	GLY	GLY	LEU	GLY	GLY	TYR	MET	LEU
12	GLY	SER	ALA	MET	SER	ARG	PRO	ILE	ILE	HIS
13	PHE	GLY	SER	ASP	TYR	GLU	ASP	ARG	TYR	TYR
14	ARG	GLU	ASN	MET	HIS	ARG	TYR	PRO	ASN	GLN
15	VAL	TYR	TYR	ARG	PRO	MET	ASP	GLU	TYR	SER
16	ASN	GLN	ASN	ASN	PHE	VAL	HIS	ASP	CYS	VAL
17	ASN	ILE	THR	ILE	LYS	GLN	HIS	THR	VAL	THR
18	THR	THR	THR	LYS	GLY	GLU	ASN	PHE	THR	GLU
19	THR	ASP	VAL	LYS	MET	MET	GLU	ARG	VAL	VAL
20	GLU	GLN	MET	CYS	ILE	THR	GLN	TYR	GLU	ARG
21	GLU	SER	GLN	ALA	TYR	TYR	GLN	ARG	GLY	SER

Samples:

sample_1: human prion protein, [U-13C; U-15N], 1 mM

Ex-cond1: ionic strength: 0.01 M; pH: 4.5; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	not available	not available	not available
ct-HNCA	not available	not available	not available
HNCACB	not available	not available	not available
CBCA(CO)NNH	not available	not available	not available
HN(COCA)NNH	not available	not available	not available
(H)N(CO-TOCSY)NH	not available	not available	not available
(H)CA(CO-TOCSY)NH	not available	not available	not available
(H)CBCA(CO-TOCSY)NH	not available	not available	not available

Software:

No software information available

NMR spectrometers:

Bruker DRX 600 MHz

BMRB	16743 16757 17714 17756 17757 17780 18426 18550 19268 4379 4434 4620 4641 4736
PDB	1FKC 1FO7 1H0L 1I4M 1QLX 1QLZ 1QM0 1QM1 1QM2 1QM3 2KUN 2LEJ 2LFT 2LSB 2LV1 2M8T 2W9E 3HAF 3HEQ 3HER 3HES 3HJ5 3HJX 4DGI 4KML 4N9O
DBJ	BAA00011 BAF62360 BAG32276 BAG32277 BAG32278
EMBL	CAG46836 CAG46869 CAH92912
GB	AAA19664 AAA60182 AAA68632 AAA68633 AAB59443
REF	NP_000302 NP_001009093 NP_001073590 NP_001073591 NP_001073592
SP	P04156 P40252 P40256 P61766 P61767
TPE	CAJ43808
AlphaFold	P04156 P40252 P40256 P61766 P61767

1	GLY	SER	LYS	LYS	ARG	PRO	LYS	PRO	GLY	GLY
2	TRP	ASN	THR	GLY	GLY	SER	ARG	TYR	PRO	GLY
3	GLN	GLY	SER	PRO	GLY	GLY	ASN	ARG	TYR	PRO
4	PRO	GLN	GLY	GLY	GLY	GLY	TRP	GLY	GLN	PRO
5	HIS	GLY	GLY	GLY	TRP	GLY	GLN	PRO	HIS	GLY
6	GLY	GLY	TRP	GLY	GLN	PRO	HIS	GLY	GLY	GLY
7	TRP	GLY	GLN	PRO	HIS	GLY	GLY	GLY	TRP	GLY
8	GLN	GLY	GLY	GLY	THR	HIS	SER	GLN	TRP	ASN
9	LYS	PRO	SER	LYS	PRO	LYS	THR	ASN	MET	LYS
10	HIS	MET	ALA	GLY	ALA	ALA	ALA	ALA	GLY	ALA
11	VAL	VAL	GLY	GLY	LEU	GLY	GLY	TYR	MET	LEU
12	GLY	SER	ALA	MET	SER	ARG	PRO	ILE	ILE	HIS
13	PHE	GLY	SER	ASP	TYR	GLU	ASP	ARG	TYR	TYR
14	ARG	GLU	ASN	MET	HIS	ARG	TYR	PRO	ASN	GLN
15	VAL	TYR	TYR	ARG	PRO	MET	ASP	GLU	TYR	SER
16	ASN	GLN	ASN	ASN	PHE	VAL	HIS	ASP	CYS	VAL
17	ASN	ILE	THR	ILE	LYS	GLN	HIS	THR	VAL	THR
18	THR	THR	THR	LYS	GLY	GLU	ASN	PHE	THR	GLU
19	THR	ASP	VAL	LYS	MET	MET	GLU	ARG	VAL	VAL
20	GLU	GLN	MET	CYS	ILE	THR	GLN	TYR	GLU	ARG
21	GLU	SER	GLN	ALA	TYR	TYR	GLN	ARG	GLY	SER

1	GLY	SER	LYS	LYS	ARG	PRO	LYS	PRO	GLY	GLY
2	TRP	ASN	THR	GLY	GLY	SER	ARG	TYR	PRO	GLY
3	GLN	GLY	SER	PRO	GLY	GLY	ASN	ARG	TYR	PRO
4	PRO	GLN	GLY	GLY	GLY	GLY	TRP	GLY	GLN	PRO
5	HIS	GLY	GLY	GLY	TRP	GLY	GLN	PRO	HIS	GLY
6	GLY	GLY	TRP	GLY	GLN	PRO	HIS	GLY	GLY	GLY
7	TRP	GLY	GLN	PRO	HIS	GLY	GLY	GLY	TRP	GLY
8	GLN	GLY	GLY	GLY	THR	HIS	SER	GLN	TRP	ASN
9	LYS	PRO	SER	LYS	PRO	LYS	THR	ASN	MET	LYS
10	HIS	MET	ALA	GLY	ALA	ALA	ALA	ALA	GLY	ALA
11	VAL	VAL	GLY	GLY	LEU	GLY	GLY	TYR	MET	LEU
12	GLY	SER	ALA	MET	SER	ARG	PRO	ILE	ILE	HIS
13	PHE	GLY	SER	ASP	TYR	GLU	ASP	ARG	TYR	TYR
14	ARG	GLU	ASN	MET	HIS	ARG	TYR	PRO	ASN	GLN
15	VAL	TYR	TYR	ARG	PRO	MET	ASP	GLU	TYR	SER
16	ASN	GLN	ASN	ASN	PHE	VAL	HIS	ASP	CYS	VAL
17	ASN	ILE	THR	ILE	LYS	GLN	HIS	THR	VAL	THR
18	THR	THR	THR	LYS	GLY	GLU	ASN	PHE	THR	GLU
19	THR	ASP	VAL	LYS	MET	MET	GLU	ARG	VAL	VAL
20	GLU	GLN	MET	CYS	ILE	THR	GLN	TYR	GLU	ARG
21	GLU	SER	GLN	ALA	TYR	TYR	GLN	ARG	GLY	SER