BMRB Entry 4389

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4389

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Title:
Backbone NMR Assignment and Secondary Structure of Ribosome Recycling Factor (RRF) from Pseudomonas aeruginosa
Deposition date:
1999-09-04
Original release date:
2000-12-15
Authors:
Kashimori, Hiromasa; Yoshida, Takuya; Kijima, Hiroyuki; Shimahara, Hideto; Uchiyama, Susumu; Ishino, Tetsuya; Shuda, Masahiro; Nakano, Hiroaki; Shibata, Yasutaka; Saihara, Yukari; Ohkubo, Tadayasu; Yoshida, Toshio; Kaji, Akira; Kobayashi, Yuji
Citation:

Citation: Kashimori, Hiromasa; Yoshida, Takuya; Kijima, Hiroyuki; Shimahara, Hideto; Uchiyama, Susumu; Ishino, Tetsuya; Shuda, Masahiro; Nakano, Hiroaki; Shibata, Yasutaka; Saihara, Yukari; Ohkubo, Tadayasu; Yoshida, Toshio; Kaji, Akira; Kobayashi, Yuji. "Backbone NMR Assignment and Secondary Structure of Ribosome Recycling Factor (RRF) from Pseudomonas aeruginosa"  J. Biomol. NMR 15, 341-342 (1999).

Assembly members:

Assembly members:
Ribosome Recycling Factor, polymer, 185 residues, 20485 Da.

Natural source:

Natural source:   Common Name: Pseudomonas aeruginosa   Taxonomy ID: 287   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ribosome Recycling Factor: MINEIKKEAQERMGKTLEAL GHAFAKIRTGRAHPSILDSV MVSYYGADTPLRQVANVTVE DSRTLALAVFDKSMIQAVEK AIMTSDLGLNPATAGTTIRV PMPALTEETRKGYTKQARAE AEQARVSVRNIRRDALAQLK DLQKEKEISEDEERRAGDDV QKLTDKFIGEIEKALEAKEA DLMAV

Data sets:
Data typeCount
13C chemical shifts357
15N chemical shifts173
1H chemical shifts346

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RRF1

Entities:

Entity 1, RRF 185 residues - 20485 Da.

1   METILEASNGLUILELYSLYSGLUALAGLN
2   GLUARGMETGLYLYSTHRLEUGLUALALEU
3   GLYHISALAPHEALALYSILEARGTHRGLY
4   ARGALAHISPROSERILELEUASPSERVAL
5   METVALSERTYRTYRGLYALAASPTHRPRO
6   LEUARGGLNVALALAASNVALTHRVALGLU
7   ASPSERARGTHRLEUALALEUALAVALPHE
8   ASPLYSSERMETILEGLNALAVALGLULYS
9   ALAILEMETTHRSERASPLEUGLYLEUASN
10   PROALATHRALAGLYTHRTHRILEARGVAL
11   PROMETPROALALEUTHRGLUGLUTHRARG
12   LYSGLYTYRTHRLYSGLNALAARGALAGLU
13   ALAGLUGLNALAARGVALSERVALARGASN
14   ILEARGARGASPALALEUALAGLNLEULYS
15   ASPLEUGLNLYSGLULYSGLUILESERGLU
16   ASPGLUGLUARGARGALAGLYASPASPVAL
17   GLNLYSLEUTHRASPLYSPHEILEGLYGLU
18   ILEGLULYSALALEUGLUALALYSGLUALA
19   ASPLEUMETALAVAL

Samples:

sample_1: Ribosome Recycling Factor, [U-99% 2H; U-99% 15N], 1.5 mM

sample_2: Ribosome Recycling Factor, [U-99% 2H; U-99% 13C; U-99% 15N], 1.5 mM

sample_conditions_1: ionic strength: 0.8 M; pH: 5.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCnot availablenot availablesample_conditions_1
3D HNCAnot availablenot availablesample_conditions_1
3D HN(CO)CAnot availablenot availablesample_conditions_1
3D HNCOnot availablenot availablesample_conditions_1
3D HA(CA)NHnot availablenot availablesample_conditions_1
3D HA(CACO)NHnot availablenot availablesample_conditions_1
4D 1H-15N-15N-1H NOESYnot availablenot availablesample_conditions_1

Software:

PIPP v4.2.5 - peak assignments

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

DBJ BAA32345 BAK91607 BAP21725 BAP49359 BAQ38186
EMBL CAW26110 CCQ88442 CDH69697 CDH75776 CDI92077
GB AAG07041 ABJ12886 ABR84529 AEO73780 AFM63508
REF NP_252343 WP_003092390 WP_003109339 WP_012074694 WP_023097294
SP A6V1D5 B7V7G0 O82853 Q02RC5
AlphaFold A6V1D5 B7V7G0 O82853 Q02RC5

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks