BMRB Entry 4368

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4368

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Title:
Chemical Shift Assignments for A35T vnd/NK2 Mutant Homeodomain
Deposition date:
1999-07-14
Original release date:
2000-06-29
Authors:
Xiang, Bosong; Weiler, S.; Nirenberg, M; Ferretti, J.
Citation:

Citation: Xiang, Bosong; Weiler, S.; Nirenberg, M; Ferretti, J.. "Structural Basis of an Embryonically Lethal Single Ala --> Thr Mutation in the vnd/NK-2 Homeodomain"  Proc. Natl. Acad. Sci. U. S. A. 95, 7412-7416 (1998).

Assembly members:

Assembly members:
A35T vnd/NK2 homeodomain, polymer, 80 residues, 9723.7 Da.
strand 1, polymer, 16 residues, Formula weight is not available
strand 2, polymer, 16 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7215   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: drosophila not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia Coli   Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
A35T vnd/NK2 homeodomain: GSHMSDGLPNKKRKRRVLFT KAQTYELERRFRQQRYLSAP EREHLTSLIRLTPTQVKIWF QNHRYKTKRAQNEKGYEGHP
strand 1: ACAGCCACTTGACACA
strand 2: TGTGTCAAGTGGCTGT

Data sets:
Data typeCount
13C chemical shifts265
15N chemical shifts76
1H chemical shifts549

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1A35T vnd/NK21
2strand 12
3strand 23

Entities:

Entity 1, A35T vnd/NK2 80 residues - 9723.7 Da.

1   GLYSERHISMETSERASPGLYLEUPROASN
2   LYSLYSARGLYSARGARGVALLEUPHETHR
3   LYSALAGLNTHRTYRGLULEUGLUARGARG
4   PHEARGGLNGLNARGTYRLEUSERALAPRO
5   GLUARGGLUHISLEUTHRSERLEUILEARG
6   LEUTHRPROTHRGLNVALLYSILETRPPHE
7   GLNASNHISARGTYRLYSTHRLYSARGALA
8   GLNASNGLULYSGLYTYRGLUGLYHISPRO

Entity 2, strand 1 16 residues - Formula weight is not available

1   DADCDADGDCDCDADCDTDT
2   DGDADCDADCDA

Entity 3, strand 2 16 residues - Formula weight is not available

1   DTDGDTDGDTDCDADADGDT
2   DGDGDCDTDGDT

Related Database Links:

BMRB 4141
PDB 1NK2 1NK3 1QRY 1VND
EMBL CAA21410 CAA60619
GB AAA28617 AAB34960 AAF45521 ABI30961 ADJ13174
REF NP_001036253 NP_476786 XP_001354499 XP_001966310 XP_001982405
SP P22808
AlphaFold P22808

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