BMRB Entry 4367

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4367

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Title:
Backbone assignments of a Borrelia OspA mutant containing an extended single-layer beta-sheet
Deposition date:
1999-07-14
Original release date:
2000-03-23
Authors:
Huang, Xiaolin; Link, Karl; Koide, Akiko; Koide, Shohei
Citation:

Citation: Koide, Shohei; Huang, Xiaolin; Link, Karl; Koide, Akiko; Bu, Zimei; Engelman, Donald. "Design of single-layer beta-sheets without a hydrophobic core"  Nature 403, 456-460 (2000).
PubMed: 10667801

Assembly members:

Assembly members:
OspA mutant, polymer, 280 residues, 30260 Da.

Natural source:

Natural source:   Common Name: Lyme disease spirochete   Taxonomy ID: 139   Superkingdom: not available   Kingdom: not available   Genus/species: Borrelia burgdorferi

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: pET24a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
OspA mutant: AKQNVSSLDEKNSVSVDLPG EMKVLVSKEKNKDGKYDLIA TVDKLELKGTSDKNNGSGVL EGVKADKSKVKLTISDDLGQ TTLEVFKEDGKTLVSKKVTS KDKSSTEEKFNEKGELSEKK ITRADKSSTEEKFNEKGEVS EKIITRADGTRLEYTGIKSD GSGKAKEVLKGYVLEGTLTA EKTTLVVKEGTVTLSKNISK SGEVSVELNDTDSSAATKKT AAWNSGTSTLTITVNSKKTK DLVFTKENTITVQQYDSNGT KLEGSAVEITKLDEIKNALK

Data sets:
Data typeCount
13C chemical shifts813
15N chemical shifts277
1H chemical shifts277

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1OspA+1bh1

Entities:

Entity 1, OspA+1bh 280 residues - 30260 Da.

1   ALALYSGLNASNVALSERSERLEUASPGLU
2   LYSASNSERVALSERVALASPLEUPROGLY
3   GLUMETLYSVALLEUVALSERLYSGLULYS
4   ASNLYSASPGLYLYSTYRASPLEUILEALA
5   THRVALASPLYSLEUGLULEULYSGLYTHR
6   SERASPLYSASNASNGLYSERGLYVALLEU
7   GLUGLYVALLYSALAASPLYSSERLYSVAL
8   LYSLEUTHRILESERASPASPLEUGLYGLN
9   THRTHRLEUGLUVALPHELYSGLUASPGLY
10   LYSTHRLEUVALSERLYSLYSVALTHRSER
11   LYSASPLYSSERSERTHRGLUGLULYSPHE
12   ASNGLULYSGLYGLULEUSERGLULYSLYS
13   ILETHRARGALAASPLYSSERSERTHRGLU
14   GLULYSPHEASNGLULYSGLYGLUVALSER
15   GLULYSILEILETHRARGALAASPGLYTHR
16   ARGLEUGLUTYRTHRGLYILELYSSERASP
17   GLYSERGLYLYSALALYSGLUVALLEULYS
18   GLYTYRVALLEUGLUGLYTHRLEUTHRALA
19   GLULYSTHRTHRLEUVALVALLYSGLUGLY
20   THRVALTHRLEUSERLYSASNILESERLYS
21   SERGLYGLUVALSERVALGLULEUASNASP
22   THRASPSERSERALAALATHRLYSLYSTHR
23   ALAALATRPASNSERGLYTHRSERTHRLEU
24   THRILETHRVALASNSERLYSLYSTHRLYS
25   ASPLEUVALPHETHRLYSGLUASNTHRILE
26   THRVALGLNGLNTYRASPSERASNGLYTHR
27   LYSLEUGLUGLYSERALAVALGLUILETHR
28   LYSLEUASPGLUILELYSASNALALEULYS

Samples:

sample_one: OspA mutant, [U-13C; U-15N], 1.5 mM; sodium phosphate 10 mM; sodium chloride 50 mM; EDTA 0.05 mM

sample_conditions_set_1: pH: 6.0 na; temperature: 318 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

    Download HSQC peak lists in one of the following formats:
    CSV: Backbone or all simulated peaks
    SPARKY: Backbone or all simulated peaks