BMRB Entry 4322

Title:
1H Chemical Shift Assignment for the Complete Consensus V3 Loop Peptide of the Envelope Protein gp120 of HIV-1 in 20% Trifluoroethanol/Water.
Deposition date:
1999-03-19
Original release date:
2007-03-26
Authors:
Vranken, Wim; Budesinsky, Milos; Fant, Franky; Boulez, Kris; Borremans, Frans
Citation:

Citation: Vranken, Wim; Budesinsky, Milos; Fant, Franky; Boulez, Kris; Borremans, Frans. "The Complete Consensus V3 Loop Peptide of the Envelope Protein gp120 of HIV-1 Shows Pronounced Helical Character in Solution."  FEBS Lett. 374, 117-121 (1995).

Assembly members:

Assembly members:
consensus V3 loop, polymer, 35 residues, 3895.3 Da.

Natural source:

Natural source:   Common Name: HIV-1   Taxonomy ID: 11676   Superkingdom: not available   Kingdom: not available   Genus/species: Lentivirus Human immunodeficiency virus type 1

Experimental source:

Experimental source:   Production method: vendor

Entity Sequences (FASTA):

Entity Sequences (FASTA):
consensus V3 loop: CTRPNNNTRKSIHIGPGRAF YTTGEIIGDIRQAHC

Data sets:
Data typeCount
1H chemical shifts480

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Consensus V3 loop1

Entities:

Entity 1, Consensus V3 loop 35 residues - 3895.3 Da.

1   CYSTHRARGPROASNASNASNTHRARGLYS
2   SERILEHISILEGLYPROGLYARGALAPHE
3   TYRTHRTHRGLYGLUILEILEGLYASPILE
4   ARGGLNALAHISCYS

Samples:

water_sample: consensus V3 loop 4.0 mM; H2O 90%; D2O 10%

20%_TFE_sample: consensus V3 loop 3.4 mM; TFE 20%; H2O 70%; D2O 10%

lowT: pH: 2.5 na; temperature: 275 K

highT: pH: 2.5 na; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
1H magnitude COSYnot availablenot availablenot available
1H TOCSYnot availablenot availablenot available
1H NOESYnot availablenot availablenot available
1H ROESYnot availablenot availablenot available
1H CT-NOE spectranot availablenot availablenot available

Software:

PRONTO - Analysis of spectra

UXNMR - Processing of spectra

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

DBJ BAA21202 BAF35886
EMBL CAA08295 CAA08296 CAA12785 CAA12814 CAA82418
GenBank AAA19836 AAA19841 AAA19848 AAA44191 AAA44789