BMRB Entry 4308
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4308
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NMR-STAR v3 text file.
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Citation: Mulhern, Terrence; Bagley, Christopher; Gaunt, C.; Lopez, Angel; Vadas, M.; D'Andrea, Richard; Booker, Grant. "1H and 15N Chemical Shift Assignments for Domain 4 of the Common Beta-Chain
of the IL-3, IL-5 and GM-CSF Receptors" J. Biomol. NMR 14, 281-282 (1999).
Assembly members:
beta c domain 4, polymer, 102 residues, 12096 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
beta c domain 4: MIQMAPPSLNVTKDGDSYSL
RWETMKMRYEHIDHTFEIQY
RKDTATWKDSKTETLQNAHS
MALPALEPSTRYWARVRVRT
SRTGYNGIWSEWSEARSWDT
ES
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 111 |
| 1H chemical shifts | 710 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | beta c domain 4 | 1 |
Entities:
Entity 1, beta c domain 4 102 residues - 12096 Da.
| 1 | MET | ILE | GLN | MET | ALA | PRO | PRO | SER | LEU | ASN | ||||
| 2 | VAL | THR | LYS | ASP | GLY | ASP | SER | TYR | SER | LEU | ||||
| 3 | ARG | TRP | GLU | THR | MET | LYS | MET | ARG | TYR | GLU | ||||
| 4 | HIS | ILE | ASP | HIS | THR | PHE | GLU | ILE | GLN | TYR | ||||
| 5 | ARG | LYS | ASP | THR | ALA | THR | TRP | LYS | ASP | SER | ||||
| 6 | LYS | THR | GLU | THR | LEU | GLN | ASN | ALA | HIS | SER | ||||
| 7 | MET | ALA | LEU | PRO | ALA | LEU | GLU | PRO | SER | THR | ||||
| 8 | ARG | TYR | TRP | ALA | ARG | VAL | ARG | VAL | ARG | THR | ||||
| 9 | SER | ARG | THR | GLY | TYR | ASN | GLY | ILE | TRP | SER | ||||
| 10 | GLU | TRP | SER | GLU | ALA | ARG | SER | TRP | ASP | THR | ||||
| 11 | GLU | SER |
Samples:
sample_1: beta c domain 4, [U-99% 15N], 0.7 mM; phosphate 10 mM; D20 10%; H2O 90%
sample_2: beta c domain 4, [U-99% 15N], 0.7 mM; phosphate 10 mM; D2O 100%
Ex-cond_1: pH: 6.1 na; temperature: 293 K
Ex-cond_2: pH*: 6.1 na; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | not available | not available | not available |
| TOCSY | not available | not available | not available |
| DQF-COSY | not available | not available | not available |
| 1H-15N HSQC | not available | not available | not available |
| 1H-15N HMQC-J | not available | not available | not available |
| 1H-15N NOESY-HSQC | not available | not available | not available |
| 1H-15N TOCSY-HSQC | not available | not available | not available |
Software:
XEASY - assignment
NMR spectrometers:
- Varian INOVA 600 MHz
Related Database Links:
| PDB | |
| DBJ | BAG10649 BAG64130 |
| EMBL | CAG30314 CAK54420 CAK54719 |
| GB | AAA18171 AAB31055 AAH70085 EAW60125 EAW60126 |
| REF | NP_000386 XP_003821586 XP_003821587 XP_004063464 XP_004063465 |
| SP | P32927 |
| AlphaFold | P32927 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks