BMRB Entry 4215

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PDB ID: 1ssn
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4215
MolProbity Validation Chart

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Title:
Staphylokinase (NMR, Sakstar Variant)
Deposition date:
1998-06-08
Original release date:
2000-10-02
Authors:
Ohlenschlager, Oliver; Ramachandran, Ramadurai; Guhrs, Karl; Schlott, Bernhard; Brown, Larry
Citation:

Citation: Ohlenschlager, Oliver; Ramachandran, Ramadurai; Guhrs, Karl; Schlott, Bernhard; Brown, Larry. "NMR Solution Structure of the Plasminogen-Activator Protein Staphylokinase"  Biochemistry 37, 10635-10642 (1998).

Assembly members:

Assembly members:
Staphylokinase, polymer, 136 residues, 15500 Da.

Natural source:

Natural source:   Common Name: S. aureus   Taxonomy ID: 1280   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Staphylococcus aureus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Staphylokinase: SSSFDKGKYKKGDDASYFEP TGPYLMVNVTGVDSKGNELL SPHYVEFPIKPGTTLTKEKI EYYVEWALDATAYKEFRVVE LDPSAKIEVTYYDKNKKKEE TKSFPITEKGFVVPDLSEHI KNPGFNLITKVVIEKK

Data sets:
Data typeCount
13C chemical shifts629
15N chemical shifts130
1H chemical shifts987

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1Staphylokinase1

Entities:

Entity 1, Staphylokinase 136 residues - 15500 Da.

1   SERSERSERPHEASPLYSGLYLYSTYRLYS
2   LYSGLYASPASPALASERTYRPHEGLUPRO
3   THRGLYPROTYRLEUMETVALASNVALTHR
4   GLYVALASPSERLYSGLYASNGLULEULEU
5   SERPROHISTYRVALGLUPHEPROILELYS
6   PROGLYTHRTHRLEUTHRLYSGLULYSILE
7   GLUTYRTYRVALGLUTRPALALEUASPALA
8   THRALATYRLYSGLUPHEARGVALVALGLU
9   LEUASPPROSERALALYSILEGLUVALTHR
10   TYRTYRASPLYSASNLYSLYSLYSGLUGLU
11   THRLYSSERPHEPROILETHRGLULYSGLY
12   PHEVALVALPROASPLEUSERGLUHISILE
13   LYSASNPROGLYPHEASNLEUILETHRLYS
14   VALVALILEGLULYSLYS

Related Database Links:

PDB 1BUI 1C76 1C77 1C78 1C79 1SSN 2SAK
DBJ BAA95011 BAB43032 BAB58106 BAB95750 BAF68152
EMBL CAA24957 CAA29822 CAG41024 CAG43674 CBI49812
GB AAA98206 AAB84174 AAK11636 AAL82378 AAP86605
PIR PRSAK
REF NP_803403 NP_835575 WP_000919348 WP_000919349 WP_000919350
SP P15240 P68801 P68802 Q6G7Z1 Q6GFB2
AlphaFold P15240 P68801 P68802 Q6G7Z1 Q6GFB2

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