BMRB Entry 4214
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4214
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Muskett, F.; Frenkiel, T.; Feeney, J.; Freedman, R.; Carr, M.; Williamson, R.. "High resolution structure of the N-terminal domain of tissue inhibitor of
metalloproteinases-2 and characterization of its interaction site with matrix
metalloproteinase-3" J. Biol. Chem. 273, 21736-21743 (1998).
PubMed: 9705310
Assembly members:
tissue inhibitor of metalloproteinases-2 (TIMP-2), polymer, 127 residues, 14084 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
tissue inhibitor of metalloproteinases-2 (TIMP-2): CSCSPVHPQQAFCNADVVIR
TKAVSEKEVDSGNDIYGNPI
KRIQYEIKQIKMFKGPEKDI
EFIYTAPSSAVCGVSLDVGG
KKEYLIAGKAEGDGKMHITL
CDFIVPWDTLSTTQKKSLNH
RYQMGCE
- assigned_chemical_shifts
- coupling_constants
| Data type | Count |
| 13C chemical shifts | 372 |
| 15N chemical shifts | 128 |
| 1H chemical shifts | 828 |
| coupling constants | 164 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | N-TIMP-2 monomer | 1 |
Entities:
Entity 1, N-TIMP-2 monomer 127 residues - 14084 Da.
| 1 | CYS | SER | CYS | SER | PRO | VAL | HIS | PRO | GLN | GLN | ||||
| 2 | ALA | PHE | CYS | ASN | ALA | ASP | VAL | VAL | ILE | ARG | ||||
| 3 | THR | LYS | ALA | VAL | SER | GLU | LYS | GLU | VAL | ASP | ||||
| 4 | SER | GLY | ASN | ASP | ILE | TYR | GLY | ASN | PRO | ILE | ||||
| 5 | LYS | ARG | ILE | GLN | TYR | GLU | ILE | LYS | GLN | ILE | ||||
| 6 | LYS | MET | PHE | LYS | GLY | PRO | GLU | LYS | ASP | ILE | ||||
| 7 | GLU | PHE | ILE | TYR | THR | ALA | PRO | SER | SER | ALA | ||||
| 8 | VAL | CYS | GLY | VAL | SER | LEU | ASP | VAL | GLY | GLY | ||||
| 9 | LYS | LYS | GLU | TYR | LEU | ILE | ALA | GLY | LYS | ALA | ||||
| 10 | GLU | GLY | ASP | GLY | LYS | MET | HIS | ILE | THR | LEU | ||||
| 11 | CYS | ASP | PHE | ILE | VAL | PRO | TRP | ASP | THR | LEU | ||||
| 12 | SER | THR | THR | GLN | LYS | LYS | SER | LEU | ASN | HIS | ||||
| 13 | ARG | TYR | GLN | MET | GLY | CYS | GLU |
Samples:
sample_1: tissue inhibitor of metalloproteinases-2 (TIMP-2), [U-96% 13C; U-98% 15N], 1.4 mM; Sodium Phosphate 25 mM; potassium Chloride 100 mM
sample_cond_1: ionic strength: 125 mM; pH*: 6.7; pressure: 1 atm; temperature: 308 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NOESY | sample_1 | not available | sample_cond_1 |
| 2D TOCSY | sample_1 | not available | sample_cond_1 |
| 2D COSY | sample_1 | not available | sample_cond_1 |
| 3D NOESY-TOCSY | sample_1 | not available | sample_cond_1 |
| 2D HSQC | sample_1 | not available | sample_cond_1 |
| 3D 15N NOESY HSQC | sample_1 | not available | sample_cond_1 |
| 3D 15N HNHA | sample_1 | not available | sample_cond_1 |
| 3D 15N HNHB | sample_1 | not available | sample_cond_1 |
| 3D HCCH TOCSY | sample_1 | not available | sample_cond_1 |
| 3D CBCA(CO)NH | sample_1 | not available | sample_cond_1 |
| 3D CBCAHN | sample_1 | not available | sample_cond_1 |
| 3D 13C HMQC NOESY | sample_1 | not available | sample_cond_1 |
Software:
NMRPipe v7/9/97 - NMR data processing
XEASY v1.13.11 - Spectral analysis
DYANA v1.4 - Structure Calculation
NMR spectrometers:
- Varian UnityPlus 600 MHz
- Varian Inova 600 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks