BMRB Entry 4159
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4159
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Citation: Connolly, Peter; Stern, Alan; Hoch, Jeffrey. "Solution Structure of LSIII, a Long Neurotoxin from the Venom of Laticauda
semifasciata" Biochemistry 35, 418-426 (1996).
Assembly members:
Long Neurotoxin 1 (Component LSIII), polymer, 66 residues, Formula weight is not available
Natural source: Common Name: Broad-banded blue sea krait Taxonomy ID: 8631 Superkingdom: Eucaryota Kingdom: Metazoa Genus/species: Laticauda semifasciata
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
Long Neurotoxin 1 (Component LSIII): RECYLNPHDTQTCPSGQEIC
YVKSWCNAWCSSRGKVLEFG
CAATCPSVNTGTEIKCCSAD
KCNTYP
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 387 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | LSIII | 1 |
Entities:
Entity 1, LSIII 66 residues - Formula weight is not available
| 1 | ARG | GLU | CYS | TYR | LEU | ASN | PRO | HIS | ASP | THR | ||||
| 2 | GLN | THR | CYS | PRO | SER | GLY | GLN | GLU | ILE | CYS | ||||
| 3 | TYR | VAL | LYS | SER | TRP | CYS | ASN | ALA | TRP | CYS | ||||
| 4 | SER | SER | ARG | GLY | LYS | VAL | LEU | GLU | PHE | GLY | ||||
| 5 | CYS | ALA | ALA | THR | CYS | PRO | SER | VAL | ASN | THR | ||||
| 6 | GLY | THR | GLU | ILE | LYS | CYS | CYS | SER | ALA | ASP | ||||
| 7 | LYS | CYS | ASN | THR | TYR | PRO |
Samples:
sample_one: Long Neurotoxin 1 (Component LSIII) 4 mM
sample_conditions_one: pH: 4.6; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 1H-1H P.COSY | sample_one | not available | sample_conditions_one |
| 1H-1H P.E. COSY | sample_one | not available | sample_conditions_one |
| 1H-1H TOCSY | sample_one | not available | sample_conditions_one |
| 1H-1H NOESY | sample_one | not available | sample_conditions_one |
| 1H-1H RELAY | sample_one | not available | sample_conditions_one |
| 1H-1H DOUBLE-RELAY | sample_one | not available | sample_conditions_one |
| 1H-1H-1H NOESY-TOCSY | sample_one | not available | sample_conditions_one |
Software:
RNMRTK v3 - data processing
EASY - peak assignment
NMR spectrometers:
- JEQL GX 400 MHz
- Bruker AMX 500 MHz