BMRB Entry 4076
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4076
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Citation: Pham, Thuy-Nga; Koide, Shohei. "NMR Studies of Borrelia burgdorferi OspA, a 28 kDa
Protein Containing a Single-Layer Beta-sheet" J. Biomol. NMR 11, 407-414 (1998).
Assembly members:
Outer Surface Protein A, polymer, 257 residues, 27555 Da.
Natural source: Common Name: spirochete Taxonomy ID: 139 Superkingdom: Eubacteria Kingdom: not available Genus/species: Borrelia burgdorferi
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET9-OspA
Entity Sequences (FASTA):
Outer Surface Protein A: AKQNVSSLDEKNSVSVDLPG
EMKVLVSKEKNKDGKYDLIA
TVDKLELKGTSDKNNGSGVL
EGVKADKSKVKLTISDDLGQ
TTLEVFKEDGKTLVSKKVTS
KDKSSTEEKFNEKGEVSEKI
ITRADGTRLEYTGIKSDGSG
KAKEVLKGYVLEGTLTAEKT
TLVVKEGTVTLSKNISKSGE
VSVELNDTDSSAATKKTAAW
NSGTSTLTITVNSKKTKDLV
FTKENTITVQQYDSNGTKLE
GSAVEITKLDEIKNALK
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 1041 |
| 15N chemical shifts | 254 |
| 1H chemical shifts | 930 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | OspA | 1 |
Entities:
Entity 1, OspA 257 residues - 27555 Da.
| 1 | ALA | LYS | GLN | ASN | VAL | SER | SER | LEU | ASP | GLU | ||||
| 2 | LYS | ASN | SER | VAL | SER | VAL | ASP | LEU | PRO | GLY | ||||
| 3 | GLU | MET | LYS | VAL | LEU | VAL | SER | LYS | GLU | LYS | ||||
| 4 | ASN | LYS | ASP | GLY | LYS | TYR | ASP | LEU | ILE | ALA | ||||
| 5 | THR | VAL | ASP | LYS | LEU | GLU | LEU | LYS | GLY | THR | ||||
| 6 | SER | ASP | LYS | ASN | ASN | GLY | SER | GLY | VAL | LEU | ||||
| 7 | GLU | GLY | VAL | LYS | ALA | ASP | LYS | SER | LYS | VAL | ||||
| 8 | LYS | LEU | THR | ILE | SER | ASP | ASP | LEU | GLY | GLN | ||||
| 9 | THR | THR | LEU | GLU | VAL | PHE | LYS | GLU | ASP | GLY | ||||
| 10 | LYS | THR | LEU | VAL | SER | LYS | LYS | VAL | THR | SER | ||||
| 11 | LYS | ASP | LYS | SER | SER | THR | GLU | GLU | LYS | PHE | ||||
| 12 | ASN | GLU | LYS | GLY | GLU | VAL | SER | GLU | LYS | ILE | ||||
| 13 | ILE | THR | ARG | ALA | ASP | GLY | THR | ARG | LEU | GLU | ||||
| 14 | TYR | THR | GLY | ILE | LYS | SER | ASP | GLY | SER | GLY | ||||
| 15 | LYS | ALA | LYS | GLU | VAL | LEU | LYS | GLY | TYR | VAL | ||||
| 16 | LEU | GLU | GLY | THR | LEU | THR | ALA | GLU | LYS | THR | ||||
| 17 | THR | LEU | VAL | VAL | LYS | GLU | GLY | THR | VAL | THR | ||||
| 18 | LEU | SER | LYS | ASN | ILE | SER | LYS | SER | GLY | GLU | ||||
| 19 | VAL | SER | VAL | GLU | LEU | ASN | ASP | THR | ASP | SER | ||||
| 20 | SER | ALA | ALA | THR | LYS | LYS | THR | ALA | ALA | TRP | ||||
| 21 | ASN | SER | GLY | THR | SER | THR | LEU | THR | ILE | THR | ||||
| 22 | VAL | ASN | SER | LYS | LYS | THR | LYS | ASP | LEU | VAL | ||||
| 23 | PHE | THR | LYS | GLU | ASN | THR | ILE | THR | VAL | GLN | ||||
| 24 | GLN | TYR | ASP | SER | ASN | GLY | THR | LYS | LEU | GLU | ||||
| 25 | GLY | SER | ALA | VAL | GLU | ILE | THR | LYS | LEU | ASP | ||||
| 26 | GLU | ILE | LYS | ASN | ALA | LEU | LYS |
Samples:
sample_one: Outer Surface Protein A, [U-99% 13C; U-98% 15N], 1.5 mM; sodium phosphate 10 mM; sodium chloride 50 mM; EDTA 0.05 mM
sample_conditions: ionic strength: 60 mM; pH: 6.0; pressure: 1.0 atm; temperature: 318 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks