BMRB Entry 4035

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4035

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of the DNA-Binding Domain of a Human Papillomavirus E2 Protein: Evidence for Flexible DNA-Binding Regions

Deposition date:

1997-06-16

Original release date:

2005-04-21

Authors:

Liang, Heng; Petros, Andrew; Meadows, Robert; Yoon, Ho; Egan, David; Walter, Karl; Holzman, Thomas; Robins, Terry; Fesik, Stephen

Citation:

Citation: Liang, Heng; Petros, Andrew; Meadows, Robert; Yoon, Ho; Egan, David; Walter, Karl; Holzman, Thomas; Robins, Terry; Fesik, Stephen. "Solution Structure of the DNA-Binding Domain of a Human Papillomavirus E2 Protein: Evidence for Flexible DNA-Binding Regions" Biochemistry 35, 2095-2103 (1996).

Assembly members:

Assembly members:
Regulatory protein E2, polymer, 83 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Papillomavirus Taxonomy ID: 10566 Superkingdom: not available Kingdom: not available Genus/species: Papillomavirus human papillomavirus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-3b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Regulatory protein E2: MATTPIIHLKGDANILKCLR YRLSKYKQLYEQVSSTWHWT CTDGKHKNAIVTLTYISTSQ RDDFLNTVKIPNTVSVSTGY MTI

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_one

Data type	Count
13C chemical shifts	296
15N chemical shifts	84
1H chemical shifts	599

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	E2-HPV	1

Entities:

Entity 1, E2-HPV 83 residues - Formula weight is not available

1

MET

ALA

THR

PRO

ILE

HIS

LEU

LYS

2

GLY

ASP

ALA

ASN

ILE

LEU

LYS

CYS

LEU

ARG

3

TYR

ARG

LEU

SER

LYS

TYR

LYS

GLN

LEU

TYR

4

GLU

GLN

VAL

SER

THR

TRP

HIS

TRP

THR

5

CYS

THR

ASP

GLY

LYS

HIS

LYS

ASN

ALA

ILE

6

VAL

THR

LEU

THR

TYR

ILE

SER

THR

SER

GLN

7

ARG

ASP

PHE

LEU

ASN

THR

VAL

LYS

ILE

8

PRO

ASN

THR

VAL

SER

VAL

SER

THR

GLY

TYR

9

MET

THR

ILE

Samples:

sample_one: Regulatory protein E2, [U-15N;U-13C], 1.7 mM; D2O 10%; H2O 90%; sodium_phosphate 20 mM; dithiothreitol 5 mM

sample_two: Regulatory protein E2, [U-15N;U-13C], 1.7 mM; D2O 100%; sodium_phosphate 20 mM; dithiothreitol 5 mM

sample_conditions_one: pH: 6.5; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

NMR spectrometers:

Bruker AMX600 600 MHz
Bruker DMX500 500 MHz
Bruker AMX500 500 MHz

PDB	1A7G 1DHM
EMBL	CRH59757 CRH70730
GB	AAA46953 AEI60921 AEI60929 AEI60937 AEI60945
REF	WP_057257005
SP	P17383
AlphaFold	P17383