BMRB Entry 4011

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR4011

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
1H and 15N Assignments and Secondary Structure of the Starch-binding Domain of Glucoamylase from Aspergillus niger
Deposition date:
1996-03-01
Original release date:
2000-11-10
Authors:
Jacks, Amanda; Sorimachi, Kay; Le Gal-Coeffet, Marie-Francoise; Williamson, Gary; Archer, David; Williamson, Michael
Citation:

Citation: Jacks, Amanda; Sorimachi, Kay; Le Gal-Coeffet, Marie-Francoise; Williamson, Gary; Archer, David; Williamson, Michael. "1H and 15N Assignments and Secondary Structure of the Starch-binding Domain of Glucoamylase from Aspergillus niger"  Eur. J. Biochem. 233, 568-578 (1995).

Assembly members:

Assembly members:
Starch binding domain, polymer, 108 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Aspergillus niger   Taxonomy ID: 5061   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Aspergillus niger

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Aspergillus niger   Vector: pIGF containing the glaA gene and a KEX2 endoproteolytic cleavage site

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Starch binding domain: CTTPTAVAVTFDLTATTTYG ENIYLVGSISQLGDWETSDG IALSADKYTSSDPLWYVTVT LPAGESFEYKFIRIESDDSV EWESDPNREYTVPQACGTST ATVTDTWR

Data typeCount
15N chemical shifts108
1H chemical shifts725

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Starch binding domain1

Entities:

Entity 1, Starch binding domain 108 residues - Formula weight is not available

1   CYSTHRTHRPROTHRALAVALALAVALTHR
2   PHEASPLEUTHRALATHRTHRTHRTYRGLY
3   GLUASNILETYRLEUVALGLYSERILESER
4   GLNLEUGLYASPTRPGLUTHRSERASPGLY
5   ILEALALEUSERALAASPLYSTYRTHRSER
6   SERASPPROLEUTRPTYRVALTHRVALTHR
7   LEUPROALAGLYGLUSERPHEGLUTYRLYS
8   PHEILEARGILEGLUSERASPASPSERVAL
9   GLUTRPGLUSERASPPROASNARGGLUTYR
10   THRVALPROGLNALACYSGLYTHRSERTHR
11   ALATHRVALTHRASPTHRTRPARG

Samples:

sample_one: Starch binding domain, [U-100% 15N], 1.0 mM; H2O 90%; D2O 10%

sample_conditions_one: pH: 5.2; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • . . 500 MHz

Related Database Links:

BMRB 11428
PDB
DBJ BAA00331 BAD06004 GAA90865
EMBL CAA25219 CAA25303 CAK38411
GB AAB02927 AAB59296 AAP04499 AAT58037 AAT67041
PRF 1008149A 1508161A
REF XP_001390530
SP P23176 P69327 P69328
AlphaFold P23176 P69327 P69328

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks