BMRB Entry 36200
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR36200
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
All files associated with the entry
Citation: Sinha, S.; Bhattacharjya, S.. "YR26_SDS" .
Assembly members:
YR26_SDS, polymer, 26 residues, 3369.87 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
YR26_SDS: YYHFWHRGVTKRSLSPHRPR
HSRLQR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 16 |
| 15N chemical shifts | 22 |
| 1H chemical shifts | 49 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity_1 | 1 |
Entities:
Entity 1, entity_1 26 residues - 3369.87 Da.
| 1 | TYR | TYR | HIS | PHE | TRP | HIS | ARG | GLY | VAL | THR | ||||
| 2 | LYS | ARG | SER | LEU | SER | PRO | HIS | ARG | PRO | ARG | ||||
| 3 | HIS | SER | ARG | LEU | GLN | ARG |
Samples:
sample_1: YR26_SDS, 15N 13C, 300 uM; H2O 90%; D2O, [U-2H], 10%
sample_conditions_1: ionic strength: 0.3 mM; pH: 7; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
Sparky, Goddard - chemical shift assignment
CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation
TopSpin, Bruker Biospin - collection
NMR spectrometers:
- Bruker DRX 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks