BMRB Entry 36101

Title:
Solution structure of the complex between UVSSA acidic region and TFIIH p62 PH domain
Deposition date:
2017-06-27
Original release date:
2018-07-11
Authors:
Okuda, M.; Nishimura, Y.
Citation:

Citation: Okuda, M.; Nakazawa, Y.; Guo, C.; Ogi, T.; Nishimura, Y.. "Common TFIIH recruitment mechanism in global genome and transcription-coupled repair subpathways"  Nucleic Acids Res. 45, 13043-13055 (2017).
PubMed: 29069470

Assembly members:

Assembly members:
entity_1, polymer, 48 residues, 5507.810 Da.
entity_2, polymer, 110 residues, 12450.445 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Data sets:
Data typeCount
13C chemical shifts662
15N chemical shifts156
1H chemical shifts1018

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 48 residues - 5507.810 Da.

1   GLYSERMETARGARGARGTHRGLUALALEU
2   GLYASPALAGLUGLUASPGLUASPASPGLU
3   ASPPHEVALGLUVALPROGLULYSGLUGLY
4   TYRGLUPROHISILEPROASPHISLEUARG
5   PROGLUTYRGLYLEUGLUALAALA

Entity 2, entity_2 110 residues - 12450.445 Da.

1   GLYSERMETALATHRSERSERGLUGLUVAL
2   LEULEUILEVALLYSLYSVALARGGLNLYS
3   LYSGLNASPGLYALALEUTYRLEUMETALA
4   GLUARGILEALATRPALAPROGLUGLYLYS
5   ASPARGPHETHRILESERHISMETTYRALA
6   ASPILELYSCYSGLNLYSILESERPROGLU
7   GLYLYSALALYSILEGLNLEUGLNLEUVAL
8   LEUHISALAGLYASPTHRTHRASNPHEHIS
9   PHESERASNGLUSERTHRALAVALLYSGLU
10   ARGASPALAVALLYSASPLEULEUGLNGLN
11   LEULEUPROLYSPHELYSARGLYSALAASN

Samples:

sample_1: TFIIH p62 0.48 mM; UVSSA, [U-99% 13C; U-99% 15N], 0.40 mM; H2O 90%; D2O, [U-2H], 10%

sample_2: TFIIH p62 0.48 mM; UVSSA, [U-99% 13C; U-99% 15N], 0.40 mM; D2O, [U-2H], 100%

sample_3: TFIIH p62, [U-99% 13C; U-99% 15N], 0.40 mM; UVSSA 0.48 mM; H2O 90%; D2O, [U-2H], 10%

sample_4: TFIIH p62, [U-99% 13C; U-99% 15N], 0.40 mM; UVSSA 0.48 mM; D2O, [U-2H], 100%

sample_conditions_1: ionic strength: 20 mM; pH: 6.80; pressure: 1 atm; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1
3D 1H-13C NOESYsample_4isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_4isotropicsample_conditions_1
2D 1H-13C HSQCsample_4isotropicsample_conditions_1
3D 1H-15N NOESYsample_3isotropicsample_conditions_1
3D H(CCO)NHsample_3isotropicsample_conditions_1
3D C(CO)NHsample_3isotropicsample_conditions_1
3D HBHA(CO)NHsample_3isotropicsample_conditions_1
3D HNCOsample_3isotropicsample_conditions_1
3D CBCA(CO)NHsample_3isotropicsample_conditions_1
3D HCCH-TOCSYsample_3isotropicsample_conditions_1
3D HNHBsample_3isotropicsample_conditions_1
3D HNHBsample_1isotropicsample_conditions_1

Software:

Molmol, Koradi, Billeter and Wuthrich - data analysis

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment

ProcheckNMR, Laskowski and MacArthur - data analysis

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure calculation

NMR spectrometers:

  • Bruker AvanceIII 950 MHz
  • Bruker AvanceIII 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks