BMRB Entry 36070

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PDB ID: 5xe4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR36070
MolProbity Validation Chart

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Title:
NMR solution structure of the aromatic mutant H43W H67F cytochrome b5
Deposition date:
2017-03-31
Original release date:
2018-02-12
Authors:
Swati, B.; Siddhartha, P.
Citation:

Citation: Balakrishnan, Swati; Sarma, Siddhartha. "Engineering Aromatic-Aromatic Interactions To Nucleate Folding in Intrinsically Disordered Regions of Proteins"  Biochemistry 56, 4346-4359 (2017).
PubMed: 28738155

Assembly members:

Assembly members:
Cytochrome b5, polymer, 98 residues, 11314.472 Da.

Natural source:

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli BL21(DE3)   Vector: pET21(a)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cytochrome b5: AEQSDKDVKYYTLEEIQKHK DSKSTWVILHHKVYDLTKFL EEWPGGEEVLREQAGGDATE NFEDVGFSTDARELSKKYII GELHPDDRSKIAKPSETL

Data sets:
Data typeCount
13C chemical shifts196
15N chemical shifts83
1H chemical shifts636

Additional metadata:

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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 98 residues - 11314.472 Da.

1   ALAGLUGLNSERASPLYSASPVALLYSTYR
2   TYRTHRLEUGLUGLUILEGLNLYSHISLYS
3   ASPSERLYSSERTHRTRPVALILELEUHIS
4   HISLYSVALTYRASPLEUTHRLYSPHELEU
5   GLUGLUTRPPROGLYGLYGLUGLUVALLEU
6   ARGGLUGLNALAGLYGLYASPALATHRGLU
7   ASNPHEGLUASPVALGLYPHESERTHRASP
8   ALAARGGLULEUSERLYSLYSTYRILEILE
9   GLYGLULEUHISPROASPASPARGSERLYS
10   ILEALALYSPROSERGLUTHRLEU

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