BMRB Entry 34893
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34893
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Muhle-Goll, C.; Moser, C.. "Solution NMR structure of Amyloid beta precursor like protein 2 TMD" to be published ., .-..
Assembly members:
entity_1, polymer, 31 residues, 3211.024 Da.
Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity_1: SLSSSALIGLLVIAVAIATV
IVISLVMLRKR
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 87 |
| 1H chemical shifts | 208 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | unit_1 | 1 |
Entities:
Entity 1, unit_1 31 residues - 3211.024 Da.
| 1 | SER | LEU | SER | SER | SER | ALA | LEU | ILE | GLY | LEU | ||||
| 2 | LEU | VAL | ILE | ALA | VAL | ALA | ILE | ALA | THR | VAL | ||||
| 3 | ILE | VAL | ILE | SER | LEU | VAL | MET | LEU | ARG | LYS | ||||
| 4 | ARG |
Samples:
sample_1: APLP2 0.5 ± 0.2 uM
sample_conditions_1: ionic strength: 0 Not defined; pH: 6.5; pressure: 1 atm; temperature: 300 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
CcpNmr Analysis v2.4.2, CCPN - chemical shift assignment, peak picking
ARIA v2.3.2, Linge, O'Donoghue and Nilges - refinement, structure calculation
NMR spectrometers:
- Bruker AVANCE III 600 MHz