BMRB Entry 34877

Title:
(3+1) hybrid-2 G-quadruplex with a -(llp) loop progression
Deposition date:
2023-11-14
Original release date:
2023-12-26
Authors:
Jana, J.; Vianney, Y.; Weisz, K.
Citation:

Citation: Jana, J.; Vianney, Y.; Weisz, K.. "Impact of loop length and duplex extensions on the design of hybrid-type G-quadruplexes"  Chem. Commun. (Camb) 60, 854-857 (2024).
PubMed: 38131370

Assembly members:

Assembly members:
entity_1, polymer, 25 residues, 7906.078 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: synthetic construct

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: TGAGGGTCAGGGTTGGGTTG GGTAA

Data sets:
Data typeCount
13C chemical shifts43
1H chemical shifts175

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 25 residues - 7906.078 Da.

1   DTDGDADGDGDGDTDCDADG
2   DGDGDTDTDGDGDGDTDTDG
3   DGDGDTDADA

Samples:

sample_1: DNA (25-MER) 1 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-13C HMBCsample_1isotropicsample_conditions_1

Software:

CcpNmr Analysis, CCPN - chemical shift assignment

Amber v18, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - structure calculation

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz