BMRB Entry 34813

Name: BRICHOS trimer
Published: 2024-06-20

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PDB ID: 8ox2
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34813
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

BRICHOS trimer

Deposition date:

2023-04-30

Original release date:

2024-06-20

Authors:

Ghosh, D.; Torres, F.; Guentert, P.; Riek, R.

Citation:

Citation: Ghosh, D.; Torres, F.; Guentert, P.; Riek, R.. "The inhibitory action of the chaperone BRICHOS against the alpha-Synuclein secondary nucleation pathway at near-atomic resolution" .

Assembly members:

Assembly members:
entity_1, polymer, 114 residues, 12007.753 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GSEHLVTTATFSIGSTGLVV YDYQQLLIAYKPAPGTCCYI MKIAPESIPSLEALTRKVHN FQMECSLQAKPAVPTSKLGQ AEGRDAGSAPSGGDPAFLGM AVSTLCGEVPLYYI

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	280
15N chemical shifts	98
1H chemical shifts	623

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1
2	unit_2	1
3	unit_3	1

Entities:

Entity 1, unit_1 114 residues - 12007.753 Da.

1

GLY

SER

GLU

HIS

LEU

VAL

THR

ALA

THR

2

PHE

SER

ILE

GLY

SER

THR

GLY

LEU

VAL

3

TYR

ASP

TYR

GLN

LEU

ILE

ALA

TYR

4

LYS

PRO

ALA

PRO

GLY

THR

CYS

TYR

ILE

5

MET

LYS

ILE

ALA

PRO

GLU

SER

ILE

PRO

SER

6

LEU

GLU

ALA

LEU

THR

ARG

LYS

VAL

HIS

ASN

7

PHE

GLN

MET

GLU

CYS

SER

LEU

GLN

ALA

LYS

8

PRO

ALA

VAL

PRO

THR

SER

LYS

LEU

GLY

GLN

9

ALA

GLU

GLY

ARG

ASP

ALA

GLY

SER

ALA

PRO

10

SER

GLY

ASP

PRO

ALA

PHE

LEU

GLY

MET

11

ALA

VAL

SER

THR

LEU

CYS

GLY

GLU

VAL

PRO

12

LEU

TYR

ILE

Samples:

sample_1: BRICHOS monomer, [U-13C; U-15N], 1 mM; potassium phosphate, none, 25 mM; NaCl, none, 25 mM; sodium azide, none, 0.01%

sample_conditions_1: ionic strength: 50 mM; pH: 6.8; pressure: 1 bar; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
NOESY	sample_1	isotropic	sample_conditions_1

Software:

CYANA v3.98.15, P. Guentert - structure calculation

NMR spectrometers:

Bruker AVANCE 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks