BMRB Entry 34721

Title:
Structure of a hybrid-type G-quadruplex with a snapback loop (hybrid 1R')
Deposition date:
2022-03-31
Original release date:
2022-06-09
Authors:
Jana, J.; Vianney, Y.; Schroeder, N.; Weisz, K.
Citation:

Citation: Jana, J.; Vianney, Y.; Schroeder, N.; Weisz, K.. "Guiding the folding of G-quadruplexes through loop residue interactions"  Nucleic Acid Res. 50, 7161-7175 (2022).
PubMed: 35758626

Assembly members:

Assembly members:
entity_1, polymer, 22 residues, 6928.366 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: 32630   Superkingdom: not available   Kingdom: not available   Genus/species: synthetic construct

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: XGCTAXGGTCAGGGTGGGTC AG

Data sets:
Data typeCount
13C chemical shifts24
1H chemical shifts140

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1unit_11

Entities:

Entity 1, unit_1 22 residues - 6928.366 Da.

1   GNGDGDCDTDABGMDGDGDTDC
2   DADGDGDGDTDGDGDGDTDC
3   DADG

Samples:

sample_1: DNA 0.3 mM

sample_conditions_1: ionic strength: 10 mM; pH: 7.0; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1

Software:

TopSpin v4.0.7, Bruker Biospin - processing

CcpNmr Analysis v2.4.2, CCPN - chemical shift assignment, data analysis

X-PLOR NIH v3.2, Schwieters, Kuszewski, Tjandra and Clore - structure calculation

Amber v16, Case, Darden, Cheatham III, Simmerling, Wang, Duke, Luo, ... and Kollman - refinement

CcpNmr Analysis, CCPN - peak picking

NMR spectrometers:

  • Bruker AVANCE NEO 600 MHz