BMRB Entry 34681

Name: Three-dimensional structure of the PGAM5 WT TMD
Published: 2022-04-29

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PDB ID: 7qam
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR34681
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Three-dimensional structure of the PGAM5 WT TMD

Deposition date:

2021-11-17

Original release date:

2022-04-29

Authors:

Silber, M.; Muhle-Goll, C.

Citation:

Citation: Siebert, V.; Silber, M.; Heuten, E.; Muhle-Goll, C.; Lemberg, M.. "Cleavage of mitochondrial homeostasis regulator PGAM5 by the intramembrane protease PARL is governed by transmembrane helix dynamics and oligomeric state" J. Biol. Chem. 298, 102321-102321 (2022).
PubMed: 35921890

Assembly members:

Assembly members:
entity_1, polymer, 35 residues, 3304.801 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: AFRQALQLAACGLAGGSAAV LFSAVAVGKPRAGGD

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	89
1H chemical shifts	193

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	unit_1	1

Entities:

Entity 1, unit_1 35 residues - 3304.801 Da.

1

ALA

PHE

ARG

GLN

ALA

LEU

GLN

LEU

ALA

2

CYS

GLY

LEU

ALA

GLY

SER

ALA

VAL

3

LEU

PHE

SER

ALA

VAL

ALA

VAL

GLY

LYS

PRO

4

ARG

ALA

GLY

ASP

Samples:

sample_1: PGAM5 WT 0.5 mM

sample_conditions_1: ionic strength: 1 Not defined; pH: 5.0; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

CNS, Brunger A. T. et.al. - refinement

ARIA, Linge, O'Donoghue and Nilges - structure calculation

CcpNmr Analysis, Vranken WF, Boucher W, Stevens TJ, Fogh RH, Pajon A, Llinas M, Ulrich EL, Markley JL, Ionides J, Laue ED - chemical shift assignment, peak picking

NMR spectrometers:

Bruker AVANCE III 600 MHz